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Sample GSM478452 Query DataSets for GSM478452
Status Public on Aug 15, 2013
Title Vector control clone V3, a
Sample type RNA
Source name Vector control clone V3
Organism Homo sapiens
Characteristics cell line: Panc1
cell type: pancreatic cancer
vector: control
Treatment protocol Cells were grown to 70-80% confluence before RNA extraction.
Growth protocol Panc1 stable cell lines were maintained in DMEM with 10% FBS.
Extracted molecule total RNA
Extraction protocol Extraction of total RNA was performed using the Ambion RNAqueous kit according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using GeneChip Scanner 3000.
Description PANC1_V3a
Data processing Robust multi-array analysis (RMA) using R.
Submission date Dec 02, 2009
Last update date Aug 16, 2013
Contact name Claude Chelala
Organization name Barts & The London School of Medicine (QMUL)
Department Institute of Cancer
Lab Centre for Molecular Oncology & Imaging
Street address Charterhouse square
City London
State/province London
ZIP/Postal code EC1M6BQ
Country United Kingdom
Platform ID GPL570
Series (2)
GSE19278 S100P is a metastasis-associated gene that facilitates transendothelial migration of pancreatic cancer cells [HG-U133_Plus_2]
GSE19281 S100P is a metastasis-associated gene that facilitates transendothelial migration of pancreatic cancer cells

Data table header descriptions
VALUE RMA-normalized intensity value (log-scale)

Data table
1007_s_at 9.68703011
1053_at 7.782132817
117_at 5.247439871
121_at 7.280845608
1255_g_at 2.66865951
1294_at 4.946719516
1316_at 3.886359153
1320_at 4.508597535
1405_i_at 3.273288973
1431_at 2.831351927
1438_at 5.424890722
1487_at 7.970565337
1494_f_at 5.301227015
1552256_a_at 10.31992752
1552257_a_at 7.318520362
1552258_at 3.838166456
1552261_at 4.410935957
1552263_at 5.733630317
1552264_a_at 7.702997697
1552266_at 3.180832767

Total number of rows: 54675

Table truncated, full table size 1212 Kbytes.

Supplementary file Size Download File type/resource
GSM478452.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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