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Sample GSM478454 Query DataSets for GSM478454
Status Public on Aug 15, 2013
Title Vector control clone V1
Sample type RNA
Source name Vector control clone V1
Organism Homo sapiens
Characteristics cell line: Panc1
cell type: pancreatic cancer
vector: control
Treatment protocol Cells were grown to 70-80% confluence before RNA extraction.
Growth protocol Panc1 stable cell lines were maintained in DMEM with 10% FBS.
Extracted molecule total RNA
Extraction protocol Extraction of total RNA was performed using the Ambion RNAqueous kit according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA (Expression Analysis Technical Manual, Affymetrix).
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome U133 Plus 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using GeneChip Scanner 3000.
Description SB_V1
Data processing Robust multi-array analysis (RMA) using R.
Submission date Dec 02, 2009
Last update date Aug 16, 2013
Contact name Claude Chelala
Organization name Barts & The London School of Medicine (QMUL)
Department Institute of Cancer
Lab Centre for Molecular Oncology & Imaging
Street address Charterhouse square
City London
State/province London
ZIP/Postal code EC1M6BQ
Country United Kingdom
Platform ID GPL570
Series (2)
GSE19278 S100P is a metastasis-associated gene that facilitates transendothelial migration of pancreatic cancer cells [HG-U133_Plus_2]
GSE19281 S100P is a metastasis-associated gene that facilitates transendothelial migration of pancreatic cancer cells

Data table header descriptions
VALUE RMA-normalized intensity value (log-scale)

Data table
1007_s_at 9.367105642
1053_at 8.616911565
117_at 4.868862663
121_at 7.569454797
1255_g_at 2.719427136
1294_at 5.125280265
1316_at 4.224330361
1320_at 4.631791271
1405_i_at 3.338198436
1431_at 3.018907016
1438_at 5.087324981
1487_at 8.589042745
1494_f_at 5.624577061
1552256_a_at 9.652019782
1552257_a_at 7.989969281
1552258_at 4.007495175
1552261_at 4.396649022
1552263_at 5.561026273
1552264_a_at 8.238120717
1552266_at 2.99594444

Total number of rows: 54675

Table truncated, full table size 1211 Kbytes.

Supplementary file Size Download File type/resource
GSM478454.CEL.gz 4.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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