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Sample GSM4797281 Query DataSets for GSM4797281
Status Public on Sep 22, 2020
Title uterine tissue_PKRASG12V_1402
Sample type SRA
Source name uterine tissue
Organism Mus musculus
Characteristics genetic background: Ahmr-Cre Pten flox/flox
strain: mixed C57BL/6 x 129
genotype: KRAS-G12V
age: 15 weeks
tissue: uterine tissue
Extracted molecule total RNA
Extraction protocol Total RNA were extracted from mouse uterine tissues that have been stored in RNAlater solution using Qiagen RNeasy Mini Kit.
Sequencing libraries were prepared using KAPA Stranded mRNA seq Kit and run in a single lane using Illumina HiSeq 4000 or NextSeq500 instruments.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NextSeq 500
Data processing Illumina Casava1.8 software used for basecalling.
Sequenced reads were processed with RNA-seq workflow in CLC Genomics Workbench version 12. Reads were mapped to mouse reference genome GRCm38 with default setting.
Gene expression is estimated with the EM estimation algorithm and reported as TPM (Transcripts Per Million)
Genome_build: GRCm38
Supplementary_files_format_and_content: TPM
Submission date Sep 21, 2020
Last update date Sep 22, 2020
Contact name Kwong-Kwok Wong
Phone 713-792-0229
Organization name UT M. D. Anderson Cancer Center
Department Gynecologic Oncology and Reproductive Medicine
Street address T4-3900, 1515 Holcombe Boulevard
City Houston
State/province TX
ZIP/Postal code 77030
Country USA
Platform ID GPL19057
Series (1)
GSE129520 Differences in gynecologic tumor development in Amhr2-Cre mice with KRASG12D or KRASG12V mutations
BioSample SAMN16232121
SRA SRX9163325

Supplementary file Size Download File type/resource
GSM4797281_PKRASG12V_1402.xlsx 3.8 Mb (ftp)(http) XLSX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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