|
| Status |
Public on Sep 11, 2013 |
| Title |
tumor-free, FIV-transduced female F4 |
| Sample type |
RNA |
| |
|
| Source name |
liver from FIV-transduced female mouse that did not develop tumors
|
| Organism |
Mus musculus |
| Characteristics |
strain: MF-1 outbred
age: 341 days
tissue: liver
gender: female
fiv-transduced: yes
mouse developed tumors: no
|
| Treatment protocol |
Liver tissue and tumors were flash frozen in liquid nitrogen and stored at -80 degrees until RNA extraction.
|
| Growth protocol |
Pregnant mice (16 days gestation) were anaesthetized using isoflurane. Fetuses were removed from the womb and FIV-based viral particles were administered to the yolk sac vein of each fetus. The fetuses were then returned to the womb and were delivered naturally. Mice were kept under normal conditions until they were sacrificed.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
F4-TF
|
| Data processing |
Array data were normalized by the GCRMA algorithm using Affymetrix Expression Console. Data were transformed to log 2 scale and a threshold of 5 was applied.
|
| |
|
| Submission date |
Dec 03, 2009 |
| Last update date |
Sep 11, 2013 |
| Contact name |
Reba Condiotti |
| E-mail(s) |
reba@hadassah.org.il
|
| Organization name |
Hadassah Medical Center
|
| Department |
Goldyne Savad Institute of Gene Therapy
|
| Street address |
P.O.B. 12000
|
| City |
Jerusalem |
| ZIP/Postal code |
91120 |
| Country |
Israel |
| |
|
| Platform ID |
GPL8321 |
| Series (1) |
| GSE19306 |
Hepatocellular carcinomas (HCC) in mice transduced in utero with feline immunodeficiency virus-based vectors (expression) |
|
