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Sample GSM4819647 Query DataSets for GSM4819647
Status Public on Feb 24, 2021
Title RNASeq of homo sapiens: mono-cultured wt microglia (21_Mi_WT_Mo)
Sample type SRA
 
Source name microglia
Organism Homo sapiens
Characteristics isolate: BIONi010-C iPSC line deposited at EBISC
age: 15-19
Sex: male
cell_line: BIONi010-C
health_state: normal
karyotype: 46,XY
Extracted molecule polyA RNA
Extraction protocol Cells were lysed and the RNA purified using the High Pure RNA Isolation Kit from Roche following the provided protocol. RNA purity was assessed using the Agilent 2100 Bioanalyzer.
Strand-specific mRNA-seq libraries were generated from 1 ug total RNA using the TruSeq Stranded mRNA library prep kit (Illumina) according to manufacturer's instructions
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
 
Description differentiation 1
Data processing Basecalling and sample de-multiplexing was performed with bcl2fastq v2.17.1.14 and default parameters
Reads were quality check with fastqc default parameters
Reads for each sample/replicate were mapped against the mouse genome assembly using STAR read aligner v2.5.2a
RefSeq annotated genes were quantified with samtools v1.5 and in-house scripts
Genome_build: hg38
Supplementary_files_format_and_content: Read counts per gene
 
Submission date Oct 06, 2020
Last update date Feb 24, 2021
Contact name Roland Schmucki
E-mail(s) roland.schmucki@roche.com
Organization name F. Hoffmann - La Roche AG
Street address Grenzacherstrase
City Basel
ZIP/Postal code 4058
Country Switzerland
 
Platform ID GPL20301
Series (1)
GSE159108 Generation and characterization of human iPSC-derived microglia-like cells for the use in drug discovery
Relations
BioSample SAMN16378733
SRA SRX9248303

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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