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Sample GSM4835429 Query DataSets for GSM4835429
Status Public on Dec 07, 2020
Title CM rep2
Sample type RNA
 
Source name peripherial blood mononuclear cells
Organism Homo sapiens
Characteristics disease state: cryptococcal meningitis patients
age: 32y
tissue: peripherial blood mononuclear cells
Extracted molecule total RNA
Extraction protocol Total RNA was extracted and purified using miRNeasy Mini Kit(Cat#217004,QIAGEN, GmBH, Germany) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).
Label Cy3
Label protocol Total RNA was amplified and labeled by Low Input Quick Amp WT Labeling Kit (Cat.# 5190-2943, Agilent technologies, Santa Clara, CA, US), following the manufacturer’s instructions. Labeled cRNA were purified by RNeasy mini kit (Cat.# 74106, QIAGEN, GmBH, Germany).
 
Hybridization protocol Each slide was hybridized with 1.65μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent technologies, Santa Clara, CA, US) in Hybridization Oven (Cat.# G2545A, Agilent technologies, Santa Clara, CA, US), according to the manufacturer’s instructions. After 17 hours hybridization, slides were washed in staining dishes (Cat.# 121, Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit(Cat.# 5188-5327, Agilent technologies, Santa Clara, CA, US), followed the manufacturer’s instructions。
Scan protocol Slides were scanned by Agilent Microarray Scanner (Cat#G2565CA, Agilent technologies, Santa Clara, CA, US) with default settings, Dye channel: Green, Scan resolution=3μm, PMT 100%, 20bit.
Description circRNAs expression in peripherial blood mononuclear cells from cryptococcal meningitis patients.
Data processing Data were extracted with Feature Extraction software 10.7 (Agilent technologies, Santa Clara, CA, US). Raw data were normalized by Quantile algorithm, limma packages in R.
 
Submission date Oct 19, 2020
Last update date Dec 07, 2020
Contact name Weihua Pan
Organization name Shanghai Changzheng Hospital
Department Department of Dermatology
Street address 415 Fengyang Road
City Shanghai
ZIP/Postal code 200003
Country China
 
Platform ID GPL22121
Series (1)
GSE159601 Expression profiles of circRNAs in peripherial blood mononuclear cells from cryptococcal meningitis and healthy individuals.

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
hsa_circ_0030384 1.619144246
hsa_circ_0039013 11.1251786
hsa_circ_0014371 6.137077449
hsa_circ_0041138 5.800480789
hsa_circ_0016986 3.761495332
hsa_circ_0031690 6.722533399
hsa_circ_0002702 7.915017786
hsa_circ_0026959 15.10667968
hsa_circ_0008027 8.123852656
hsa_circ_0082082 1.295166015
hsa_circ_0027730 4.743283907
hsa_circ_0023280 10.2603174
hsa_circ_0066099 9.840521798
hsa_circ_0075027 9.651424967
hsa_circ_0082731 8.783587994
hsa_circ_0024506 1.125709505
hsa_circ_0063181 11.5135359
hsa_circ_0058440 5.901511872
hsa_circ_0063323 10.84175879
hsa_circ_0001572 4.655712839

Total number of rows: 88750

Table truncated, full table size 2505 Kbytes.




Supplementary file Size Download File type/resource
GSM4835429_CM_2.txt.gz 23.9 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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