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Sample GSM4835432 Query DataSets for GSM4835432
Status Public on Dec 07, 2020
Title HC rep2
Sample type RNA
 
Source name peripherial blood mononuclear cells
Organism Homo sapiens
Characteristics disease state: healthy individuals
age: 35y
tissue: peripherial blood mononuclear cells
Extracted molecule total RNA
Extraction protocol Total RNA was extracted and purified using miRNeasy Mini Kit(Cat#217004,QIAGEN, GmBH, Germany) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).
Label Cy3
Label protocol Total RNA was amplified and labeled by Low Input Quick Amp WT Labeling Kit (Cat.# 5190-2943, Agilent technologies, Santa Clara, CA, US), following the manufacturer’s instructions. Labeled cRNA were purified by RNeasy mini kit (Cat.# 74106, QIAGEN, GmBH, Germany).
 
Hybridization protocol Each slide was hybridized with 1.65μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent technologies, Santa Clara, CA, US) in Hybridization Oven (Cat.# G2545A, Agilent technologies, Santa Clara, CA, US), according to the manufacturer’s instructions. After 17 hours hybridization, slides were washed in staining dishes (Cat.# 121, Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit(Cat.# 5188-5327, Agilent technologies, Santa Clara, CA, US), followed the manufacturer’s instructions。
Scan protocol Slides were scanned by Agilent Microarray Scanner (Cat#G2565CA, Agilent technologies, Santa Clara, CA, US) with default settings, Dye channel: Green, Scan resolution=3μm, PMT 100%, 20bit.
Description circRNAs expression in peripherial blood mononuclear cells from healthy individuals.
Data processing Data were extracted with Feature Extraction software 10.7 (Agilent technologies, Santa Clara, CA, US). Raw data were normalized by Quantile algorithm, limma packages in R.
 
Submission date Oct 19, 2020
Last update date Dec 07, 2020
Contact name Weihua Pan
Organization name Shanghai Changzheng Hospital
Department Department of Dermatology
Street address 415 Fengyang Road
City Shanghai
ZIP/Postal code 200003
Country China
 
Platform ID GPL22121
Series (1)
GSE159601 Expression profiles of circRNAs in peripherial blood mononuclear cells from cryptococcal meningitis and healthy individuals.

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
hsa_circ_0030384 1.244306719
hsa_circ_0039013 10.56393221
hsa_circ_0014371 6.296509443
hsa_circ_0041138 6.222935423
hsa_circ_0016986 5.363639632
hsa_circ_0031690 7.410865974
hsa_circ_0002702 7.588669418
hsa_circ_0026959 14.77537168
hsa_circ_0008027 8.035230927
hsa_circ_0082082 1.337384082
hsa_circ_0027730 5.430694681
hsa_circ_0023280 10.37765412
hsa_circ_0066099 10.13133476
hsa_circ_0075027 10.17867738
hsa_circ_0082731 8.707626363
hsa_circ_0024506 1.126627775
hsa_circ_0063181 12.05743611
hsa_circ_0058440 6.088627771
hsa_circ_0063323 10.35583155
hsa_circ_0001572 5.717408649

Total number of rows: 88750

Table truncated, full table size 2505 Kbytes.




Supplementary file Size Download File type/resource
GSM4835432_HC_2.txt.gz 24.0 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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