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Sample GSM4835433 Query DataSets for GSM4835433
Status Public on Dec 07, 2020
Title HC rep3
Sample type RNA
Source name peripherial blood mononuclear cells
Organism Homo sapiens
Characteristics disease state: healthy individuals
age: 38y
tissue: peripherial blood mononuclear cells
Extracted molecule total RNA
Extraction protocol Total RNA was extracted and purified using miRNeasy Mini Kit(Cat#217004,QIAGEN, GmBH, Germany) following the manufacturer’s instructions and checked for a RIN number to inspect RNA integration by an Agilent Bioanalyzer 2100 (Agilent technologies, Santa Clara, CA, US).
Label Cy3
Label protocol Total RNA was amplified and labeled by Low Input Quick Amp WT Labeling Kit (Cat.# 5190-2943, Agilent technologies, Santa Clara, CA, US), following the manufacturer’s instructions. Labeled cRNA were purified by RNeasy mini kit (Cat.# 74106, QIAGEN, GmBH, Germany).
Hybridization protocol Each slide was hybridized with 1.65μg Cy3-labeled cRNA using Gene Expression Hybridization Kit (Cat.# 5188-5242, Agilent technologies, Santa Clara, CA, US) in Hybridization Oven (Cat.# G2545A, Agilent technologies, Santa Clara, CA, US), according to the manufacturer’s instructions. After 17 hours hybridization, slides were washed in staining dishes (Cat.# 121, Thermo Shandon, Waltham, MA, US) with Gene Expression Wash Buffer Kit(Cat.# 5188-5327, Agilent technologies, Santa Clara, CA, US), followed the manufacturer’s instructions。
Scan protocol Slides were scanned by Agilent Microarray Scanner (Cat#G2565CA, Agilent technologies, Santa Clara, CA, US) with default settings, Dye channel: Green, Scan resolution=3μm, PMT 100%, 20bit.
Description circRNAs expression in peripherial blood mononuclear cells from healthy individuals.
Data processing Data were extracted with Feature Extraction software 10.7 (Agilent technologies, Santa Clara, CA, US). Raw data were normalized by Quantile algorithm, limma packages in R.
Submission date Oct 19, 2020
Last update date Dec 07, 2020
Contact name Weihua Pan
Organization name Shanghai Changzheng Hospital
Department Department of Dermatology
Street address 415 Fengyang Road
City Shanghai
ZIP/Postal code 200003
Country China
Platform ID GPL22121
Series (1)
GSE159601 Expression profiles of circRNAs in peripherial blood mononuclear cells from cryptococcal meningitis and healthy individuals.

Data table header descriptions
VALUE Normalized signal intensity

Data table
hsa_circ_0030384 1.204134664
hsa_circ_0039013 10.93843614
hsa_circ_0014371 6.367352135
hsa_circ_0041138 6.669609765
hsa_circ_0016986 4.618306696
hsa_circ_0031690 6.716149946
hsa_circ_0002702 8.476156316
hsa_circ_0026959 14.9006383
hsa_circ_0008027 8.473336134
hsa_circ_0082082 1.204134664
hsa_circ_0027730 3.492771961
hsa_circ_0023280 10.47343704
hsa_circ_0066099 9.901449201
hsa_circ_0075027 9.744828317
hsa_circ_0082731 9.256361758
hsa_circ_0024506 1.976236959
hsa_circ_0063181 11.52252971
hsa_circ_0058440 6.413553099
hsa_circ_0063323 10.75024848
hsa_circ_0001572 4.43735293

Total number of rows: 88750

Table truncated, full table size 2505 Kbytes.

Supplementary file Size Download File type/resource
GSM4835433_HC_3.txt.gz 23.7 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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