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Sample GSM4853227 Query DataSets for GSM4853227
Status Public on Jan 24, 2021
Title pilot_W083_H2O_multi_R2
Sample type SRA
 
Source name Negative Control
Organism synthetic construct
Characteristics sample type: Negative Control
Extracted molecule total RNA
Extraction protocol Total RNA extraction by Norgen total RNA Kit or RNA-XP beads.
C19-SparSeq-Barcoded multiplexed PCR reactions were pooled and cleaned with SPRIselect beads.
 
Library strategy OTHER
Library source transcriptomic
Library selection other
Instrument model NextSeq 550
 
Description SparSeq multiplexed PCR library
Pilot_ReadCount_Table.csv
Data processing Single-end reads mapped to amplicon sequences using Bowtie and allowing 3 mismatch for mapped read count / amplicon quantification.
Genome_build: Custom genome consists of amplicons targeted in GRCh38 and Sars-CoV-2 (Wuhan-Hu-1).
Supplementary_files_format_and_content: Sample name/ read count mapped to each targeted amplicon.
 
Submission date Oct 25, 2020
Last update date Jan 24, 2021
Contact name Jeff Wrana
E-mail(s) wrana@lunenfeld.ca
Organization name Lunenfeld Tanenbaum Research Institute
Street address 600 Unv Ave
City Toronto
State/province ON
ZIP/Postal code M5G 1X5
Country Canada
 
Platform ID GPL27609
Series (2)
GSE160033 A Multiplexed, Next-Generation Sequencing Platform for High-Throughput Detection of SARS-CoV-2 [pilot cohort]
GSE160036 A Multiplexed, Next-Generation Sequencing Platform for High-Throughput Detection of SARS-CoV-2
Relations
BioSample SAMN16540919
SRA SRX9354551

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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