|
| Status |
Public on Sep 16, 2021 |
| Title |
Tumour cell line 66c14 sample 2 |
| Sample type |
SRA |
| |
|
| Source name |
Tumour cell line
|
| Organism |
Mus musculus |
| Characteristics |
cell line: 66c14
|
| Treatment protocol |
none
|
| Growth protocol |
none
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted.
Illumina's TruSeq RNA v2 sample preparation protocol was used.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
Cell line 66c14 2
|
| Data processing |
Libraries were sequenced on an Illumina NextSeq 500.
Reads were aligned to the mouse genome mm10 by Rsubread version 1.22.3.
Read counts were summarized by Entrez Gene ID using the Rsubread featureCounts function and Rsubread's inbuilt RefSeq gene annotation.
Genome_build: mm10
Supplementary_files_format_and_content: tab-delimited text files; columns represent Entrez Gene ID, gene length (total base count of all exons) and the number of reads mapped to that gene.
|
| |
|
| Submission date |
Oct 26, 2020 |
| Last update date |
Sep 16, 2021 |
| Contact name |
Gordon K Smyth |
| E-mail(s) |
smyth@wehi.edu.au
|
| Phone |
(+61 3) 9345 2326
|
| Fax |
(+61 3) 9347 0852
|
| URL |
http://www.wehi.edu.au
|
| Organization name |
Walter and Eliza Hall Institute of Medical Research
|
| Department |
Bioinformatics
|
| Lab |
Smyth
|
| Street address |
1G Royal Pde
|
| City |
Parkville |
| State/province |
Vic |
| ZIP/Postal code |
3052 |
| Country |
Australia |
| |
|
| Platform ID |
GPL19057 |
| Series (2) |
| GSE160101 |
RNA-seq expression profiling of murine breast tumor cell lines |
| GSE160102 |
RNA-seq expression profiling of murine breast tumour cell lines and bone metastasis-associated endothelial cells |
|
| Relations |
| BioSample |
SAMN16552259 |
| SRA |
SRX9363599 |
