|
| Status |
Public on Feb 25, 2021 |
| Title |
Cd4CrePofut1_WT 2 |
| Sample type |
RNA |
| |
|
| Source name |
empty vector-transduced WT P14 cells at day 7.5 post-infection from spleen
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
transduction: empty vector
genotype/variation: WT
|
| Treatment protocol |
Sorted cells were lysed with lysis buffer from Rneasy Micro kit from Qiagen.
|
| Growth protocol |
WT or Pofut1-null P14 cells with or without NICD co-expression were sorted from spleen after LCMV-Armstrong infection.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA samples were prepared according to the prototol of Rneasy Micro kit from Qiagen,
|
| Label |
biotin
|
| Label protocol |
Total RNA (1 - 20 ng) were converted into biotinylated cDNA using the NuGEN WTA Pico v2 protocol and the NuGEN Encore Biotin module
|
| |
|
| Hybridization protocol |
Six micrograms of biotinylated cDNA was fragmented and then hybridized for 16 hr at 45C on an Affymetrix Clariom S mouse GeneChip. Following hybridization, GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the AffymetrixGeneChip Scanner 3000 7G.
|
| Data processing |
Probe signals were quantile normalized and summarized by the RMA algorithm using the Affymetrix Expression Console software v1.1
|
| |
|
| Submission date |
Oct 27, 2020 |
| Last update date |
Feb 27, 2021 |
| Contact name |
Hongbo Chi |
| E-mail(s) |
hongbo.chi@stjude.org
|
| Organization name |
St Jude Children's Research Hospital
|
| Department |
Immunology
|
| Street address |
262 Danny Thomas Place
|
| City |
Memphis |
| State/province |
TN |
| ZIP/Postal code |
38105 |
| Country |
USA |
| |
|
| Platform ID |
GPL23038 |
| Series (2) |
| GSE160225 |
In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions [Pofut1_microarray] |
| GSE160341 |
In vivo CRISPR screening reveals nutrient signaling processes underpinning CD8+ T cell fate decisions |
|
