|
| Status |
Public on Nov 10, 2020 |
| Title |
LBW male liver, biological rep2 |
| Sample type |
RNA |
| |
|
| Source name |
guinea pig liver at postnatal day 150
|
| Organism |
Cavia porcellus |
| Characteristics |
strain: Dunkin Hartley
gender: Male
treatment: Uterine artery ablation-induced low birth weight (LBW)
age: postnatal day 150
tissue: Liver
|
| Treatment protocol |
At postnatal day 150, offspring of each sex were fasted overnight, euthanized via CO2 inhalation in the morning (~ 10 am) of the following day and tissue collection conducted. The whole liver was removed and weighed immediately and the right lobe liver was frozen in liquid nitrogen and stored at −80 °C.
|
| Growth protocol |
Uterine artery ablation-induced low birth weight (LBW) and normal birth weight (NBW) male and female guinea pig offspring were fed a control diet from weaning until postnatal day 150.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA in livers was performed using the RNeasy Plus Universal Mini Kit (Qiagen) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Sense-strand cDNA was synthesized from 100 ng of total RNA, and fragmentation and labeling were performed to produce ss-cDNA with the GeneChip® WT Terminal Labeling Kit according to manufacturer’s instructions (Thermo Fisher Scientific).
|
| |
|
| Hybridization protocol |
After fragmentation and labeling, 2.1 µg cDNA was hybridized onto Guinea Pig Gene 1.1 ST Array Plate (Thermo Fisher Scientific).
|
| Scan protocol |
GeneChips were scanned using the GeneTitan® Instrument (Thermo Fisher Scientific).
|
| Description |
22_TRT004D05D05_01
Gene expression data from young adult offspring fed a control diet
|
| Data processing |
The data were analyzed with Transcriptome Analysis Console (TAC) (version 4.0, Life Technologies) using Affymetrix default analysis settings and Summarization Method as normalization method.
|
| |
|
| Submission date |
Nov 09, 2020 |
| Last update date |
Nov 10, 2020 |
| Contact name |
Ousseynou Sarr |
| E-mail(s) |
osarr@uwo.ca
|
| Phone |
2262685936
|
| Organization name |
Western universitry
|
| Department |
Physiology & Pharmacology
|
| Street address |
1811 Creekside Street
|
| City |
London |
| State/province |
United States |
| ZIP/Postal code |
N5X4L8 |
| Country |
Canada |
| |
|
| Platform ID |
GPL29378 |
| Series (1) |
| GSE161124 |
Expression Data from Young Uteroplacental Insufficiency-induced Low Birth Weight Adult Guinea Pig Offspring |
|
