|
| Status |
Public on Jun 14, 2010 |
| Title |
ADF_K27_2_(293003-2) |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Amplified H3K27me3 ChIP.
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
tissue: root epidermis hair cell nuclei
cell line: ADF8p:NTF/ACT2p:BirA
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Amplification of ChIP DNA was done with Sigma WGA4 kit. Detailed protocol is located on the Series record.
|
| Label |
Cy5
|
| Label protocol |
Standard NimbleGen protocol using 5 hour labeling reaction rather than 2 hour reaction.
|
| |
|
| Channel 2 |
| Source name |
Amplified H3 ChIP.
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
cell line: ADF8p:NTF/ACT2p:BirA
tissue: root epidermis hair cell nuclei
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Amplification of ChIP DNA was done with Sigma WGA4 kit. Detailed protocol is located on the Series record.
|
| Label |
Cy3
|
| Label protocol |
Standard NimbleGen protocol using 5 hour labeling reaction rather than 2 hour reaction.
|
| |
|
| |
| Hybridization protocol |
Standard NimbleGen protocol.
|
| Scan protocol |
Standard NimbleGen protocol.
|
| Description |
Chromatin immunoprecipitation (ChIP) profiling of the H3K27me3 modification in INTACT-purified nuclei from Arabidopsis root hair cells.
|
| Data processing |
Standard deviates of bi-weight mean adjusted log base 2 ratios of Cy5/Cy3 were computed.
|
| |
|
| Submission date |
Dec 24, 2009 |
| Last update date |
Apr 11, 2012 |
| Contact name |
Jorja Henikoff |
| E-mail(s) |
jorja@fhcrc.org
|
| Phone |
206-667-4850
|
| Organization name |
Fred Hutchinson Cancer Research Center
|
| Department |
Basic Sciences
|
| Lab |
Henikoff
|
| Street address |
1100 Fairview AV N, A1-162
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109-1024 |
| Country |
USA |
| |
|
| Platform ID |
GPL7143 |
| Series (1) |
| GSE19654 |
INTACT method for gene expression and chromatin profiling of individual cell types within a tissue |
|
