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Sample GSM4916154 Query DataSets for GSM4916154
Status Public on Nov 20, 2023
Title WT4
Sample type SRA
Source name hepatocytes
Organism Mus musculus
Characteristics cell type: liver-derived primary hepatocytes
strain: C57BL/6
genotype: wild type
tissue: liver
treatment: treated with FAC for 24 hours
Treatment protocol Liver-derived WT and Fxr-/- mouse primary hepatocytes were cultured in 10% FBS containing DMEM and treated with FAC (100μM) or AC(100μM) for 24 h.
Growth protocol 10% FBS containing DMEM at 37°C with 5%CO2
Extracted molecule total RNA
Extraction protocol RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using BGISEQ-500 standard protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model BGISEQ-500
Data processing BGISEQ-500 used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using SOAPnuke v1.4.0 with parameters -l 5 -q 0.5 -n 0.1
Fragments Per Kilobase Million (FPKM) was calculated based on the RSEM
Genome_build: GCF_000001635.26_GRCm38.p6
Supplementary_files_format_and_content: tab-delimited text file include TPM, count, and FPKM values for each Sample
Submission date Nov 20, 2020
Last update date Nov 20, 2023
Contact name Lei Cao
Organization name The School of Pharmaceutical Science and Technology (SPST)
Street address 92 Weijin Road
City Tianjin
ZIP/Postal code 300072
Country China
Platform ID GPL23479
Series (1)
GSE161863 Effects of iron overload on primary hepatocyte of WT and Fxr-/- mice
BioSample SAMN16845342
SRA SRX9536955

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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