|
| Status |
Public on Nov 20, 2023 |
| Title |
WT6 |
| Sample type |
SRA |
| |
|
| Source name |
hepatocytes
|
| Organism |
Mus musculus |
| Characteristics |
cell type: liver-derived primary hepatocytes
strain: C57BL/6
genotype: wild type
tissue: liver
treatment: treated with FAC for 24 hours
|
| Treatment protocol |
Liver-derived WT and Fxr-/- mouse primary hepatocytes were cultured in 10% FBS containing DMEM and treated with FAC (100μM) or AC(100μM) for 24 h.
|
| Growth protocol |
10% FBS containing DMEM at 37°C with 5%CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using BGISEQ-500 standard protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Data processing |
BGISEQ-500 used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using SOAPnuke v1.4.0 with parameters -l 5 -q 0.5 -n 0.1
Fragments Per Kilobase Million (FPKM) was calculated based on the RSEM
Genome_build: GCF_000001635.26_GRCm38.p6
Supplementary_files_format_and_content: tab-delimited text file include TPM, count, and FPKM values for each Sample
|
| |
|
| Submission date |
Nov 20, 2020 |
| Last update date |
Nov 20, 2023 |
| Contact name |
Lei Cao |
| E-mail(s) |
chalelab@126.com
|
| Organization name |
The School of Pharmaceutical Science and Technology (SPST)
|
| Street address |
92 Weijin Road
|
| City |
Tianjin |
| ZIP/Postal code |
300072 |
| Country |
China |
| |
|
| Platform ID |
GPL23479 |
| Series (1) |
| GSE161863 |
Effects of iron overload on primary hepatocyte of WT and Fxr-/- mice |
|
| Relations |
| BioSample |
SAMN16845340 |
| SRA |
SRX9536957 |
