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Sample GSM4916157 Query DataSets for GSM4916157
Status Public on Nov 20, 2023
Title KO1
Sample type SRA
 
Source name hepatocytes
Organism Mus musculus
Characteristics cell type: liver-derived primary hepatocytes
strain: C57BL/6
genotype: Fxr-/-
tissue: liver
treatment: treated with AC for 24 hours
Treatment protocol Liver-derived WT and Fxr-/- mouse primary hepatocytes were cultured in 10% FBS containing DMEM and treated with FAC (100μM) or AC(100μM) for 24 h.
Growth protocol 10% FBS containing DMEM at 37°C with 5%CO2
Extracted molecule total RNA
Extraction protocol RNA was harvested using Trizol reagent.
RNA libraries were prepared for sequencing using BGISEQ-500 standard protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model BGISEQ-500
 
Data processing BGISEQ-500 used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using SOAPnuke v1.4.0 with parameters -l 5 -q 0.5 -n 0.1
Fragments Per Kilobase Million (FPKM) was calculated based on the RSEM
Genome_build: GCF_000001635.26_GRCm38.p6
Supplementary_files_format_and_content: tab-delimited text file include TPM, count, and FPKM values for each Sample
 
Submission date Nov 20, 2020
Last update date Nov 20, 2023
Contact name Lei Cao
E-mail(s) chalelab@126.com
Organization name The School of Pharmaceutical Science and Technology (SPST)
Street address 92 Weijin Road
City Tianjin
ZIP/Postal code 300072
Country China
 
Platform ID GPL23479
Series (1)
GSE161863 Effects of iron overload on primary hepatocyte of WT and Fxr-/- mice
Relations
BioSample SAMN16845339
SRA SRX9536958

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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