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Sample GSM491932 Query DataSets for GSM491932
Status Public on Jun 01, 2010
Title RPaSMC exposed to GSNO for 4 hours, microarray1
Sample type RNA
 
Source name Rat pulmonary artery smooth muscle cells (RPaSMC) exposed to GSNO for 4 hours
Organism Rattus norvegicus
Characteristics strain: Sprague-Dawley
tissue: Pulmonary artery smooth muscle cells
Treatment protocol Rat pulmonary artery smooth muscle cells were either untreated or exposed to GSNO (100 mmol/L) for 1, 2, and 4 hours.
Growth protocol Cultures of primary rat pulmonary artery smooth muscle cells (RPaSMC) were prepared from explants of endothelium- and adventitia-stripped pulmonary arteries of adult Sprague-Dawley rats, as described previously [Takata, M. et al., Am J Physiol Lung Cell Mol Physiol., 280, L272-282, 2001].
Extracted molecule total RNA
Extraction protocol Total RNA from RPaSMC was extracted using Trizol® reagent (Invitrogen) according to the manufacturer’s protocol.
Label biotin
Label protocol Biotin-labeled cRNA was generated from the double-stranded cDNA by in vitro transcription using the ENZO Bioarray High Yield transcript labeling method (Affymetrix Inc., Santa Clara, CA).
 
Hybridization protocol Labeled cRNA (40 mg) was fragmented in fragmentation buffer (40 mmol/L Tris, pH 8.1, 100 mmol/L potassium acetate, 30 mmol/L magnesium acetate) for 35 min at 94oC. Following confirmation of cRNA quality by hybridization to a test chip (Affymetrix), fragmented cRNA was hybridized to Affymetrix rat U-34A GeneChips containing 8798 gene elements in the GeneChip Fluidics Station 450.
Scan protocol Gene Chips were scanned using the GeneChip® Scanner 3000.
Description Gene expression data of rat pulmonary artery smooth muscle cells exposed to GSNO for 4 hours.
Data processing Affymetrix GeneChip 5.0 software was used for scanning and data analysis according to Affymetrix protocols.
 
Submission date Dec 30, 2009
Last update date Dec 30, 2009
Contact name Andrea U Steinbicker
E-mail(s) asteinbicker@partners.org
Organization name Massachusetts General Hospital
Street address 55 Fruit Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platform ID GPL85
Series (1)
GSE19710 Microarray analysis of rat pulmonary artery smooth muscle cells before or after exposure to S-nitrosoglutathione (GSNO)

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 8.7858 A 0.891021
AFFX-MurIL10_at 20.9496 A 0.470241
AFFX-MurIL4_at 5.34556 A 0.876428
AFFX-MurFAS_at 29.8897 A 0.313723
AFFX-BioB-5_at 230.816 M 0.050229
AFFX-BioB-M_at 422.788 P 0.002867
AFFX-BioB-3_at 144.558 P 0.0219019
AFFX-BioC-5_at 823.935 P 0.000126798
AFFX-BioC-3_at 537.478 P 8.14279e-05
AFFX-BioDn-5_at 658.05 P 0.000753643
AFFX-BioDn-3_at 4548.69 P 0.000146581
AFFX-CreX-5_at 6966.48 P 4.42873e-05
AFFX-CreX-3_at 12824 P 4.42873e-05
AFFX-BioB-5_st 56.8654 A 0.275146
AFFX-BioB-M_st 73.2424 A 0.313723
AFFX-BioB-3_st 20.2634 A 0.783476
AFFX-BioC-5_st 8.17267 A 0.987453
AFFX-BioC-3_st 4.91546 A 0.814869
AFFX-BioDn-5_st 35.0027 A 0.354453
AFFX-BioDn-3_st 177.692 P 0.036569

Total number of rows: 8799

Table truncated, full table size 284 Kbytes.




Supplementary file Size Download File type/resource
GSM491932.CEL.gz 1.9 Mb (ftp)(http) CEL
GSM491932.CHP.gz 2.8 Mb (ftp)(http) CHP
GSM491932.EXP.gz 480 b (ftp)(http) EXP
Processed data included within Sample table
Processed data provided as supplementary file

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