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Sample GSM491935 Query DataSets for GSM491935
Status Public on Jun 01, 2010
Title RPaSMC exposed to GSNO for 2 hours, microarray2
Sample type RNA
 
Source name Rat pulmonary artery smooth muscle cells (RPaSMC) exposed to GSNO for 2 hours
Organism Rattus norvegicus
Characteristics strain: Sprague-Dawley
tissue: Pulmonary artery smooth muscle cells
Treatment protocol Rat pulmonary artery smooth muscle cells were either untreated or exposed to GSNO (100 mmol/L) for 1, 2, and 4 hours.
Growth protocol Cultures of primary rat pulmonary artery smooth muscle cells (RPaSMC) were prepared from explants of endothelium- and adventitia-stripped pulmonary arteries of adult Sprague-Dawley rats, as described previously [Takata, M. et al., Am J Physiol Lung Cell Mol Physiol., 280, L272-282, 2001].
Extracted molecule total RNA
Extraction protocol Total RNA from RPaSMC was extracted using Trizol® reagent (Invitrogen) according to the manufacturer’s protocol.
Label biotin
Label protocol Biotin-labeled cRNA was generated from the double-stranded cDNA by in vitro transcription using the ENZO Bioarray High Yield transcript labeling method (Affymetrix Inc., Santa Clara, CA).
 
Hybridization protocol Labeled cRNA (40 mg) was fragmented in fragmentation buffer (40 mmol/L Tris, pH 8.1, 100 mmol/L potassium acetate, 30 mmol/L magnesium acetate) for 35 min at 94oC. Following confirmation of cRNA quality by hybridization to a test chip (Affymetrix), fragmented cRNA was hybridized to Affymetrix rat U-34A GeneChips containing 8798 gene elements in the GeneChip Fluidics Station 450.
Scan protocol Gene Chips were scanned using the GeneChip® Scanner 3000.
Description Gene expression data of rat pulmonary artery smooth muscle cells exposed to GSNO for 2 hours.
Data processing Affymetrix GeneChip 5.0 software was used for scanning and data analysis according to Affymetrix protocols.
 
Submission date Dec 30, 2009
Last update date Dec 30, 2009
Contact name Andrea U Steinbicker
E-mail(s) asteinbicker@partners.org
Organization name Massachusetts General Hospital
Street address 55 Fruit Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platform ID GPL85
Series (1)
GSE19710 Microarray analysis of rat pulmonary artery smooth muscle cells before or after exposure to S-nitrosoglutathione (GSNO)

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 8.21905 A 0.883887
AFFX-MurIL10_at 8.60868 A 0.645547
AFFX-MurIL4_at 15.5626 A 0.712257
AFFX-MurFAS_at 14.4404 A 0.617401
AFFX-BioB-5_at 147.389 A 0.287743
AFFX-BioB-M_at 127.177 A 0.102165
AFFX-BioB-3_at 117.318 A 0.0726999
AFFX-BioC-5_at 388.8 P 0.0020226
AFFX-BioC-3_at 158.239 P 0.0103167
AFFX-BioDn-5_at 253.782 P 0.00556451
AFFX-BioDn-3_at 1971.86 P 0.00255552
AFFX-CreX-5_at 2092.06 P 5.16732e-05
AFFX-CreX-3_at 3866.09 P 4.42873e-05
AFFX-BioB-5_st 45.1508 A 0.300606
AFFX-BioB-M_st 73.7358 A 0.425962
AFFX-BioB-3_st 20.2847 A 0.843268
AFFX-BioC-5_st 18.1725 A 0.941556
AFFX-BioC-3_st 7.60735 A 0.949771
AFFX-BioDn-5_st 97.476 A 0.354453
AFFX-BioDn-3_st 54.7476 A 0.313723

Total number of rows: 8799

Table truncated, full table size 284 Kbytes.




Supplementary file Size Download File type/resource
GSM491935.CEL.gz 2.1 Mb (ftp)(http) CEL
GSM491935.CHP.gz 3.1 Mb (ftp)(http) CHP
GSM491935.EXP.gz 487 b (ftp)(http) EXP
Processed data included within Sample table
Processed data provided as supplementary file

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