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Sample GSM493586 Query DataSets for GSM493586
Status Public on Sep 01, 2010
Title WF_Grotewold_020907
Sample type genomic
Source name FLP/MYB88 ChIP DNA from wild type (Col) shoots (rep 2)
Organism Arabidopsis thaliana
Characteristics age: Ten-day old Green tissue
tissue: Shoots
antibody: FLP/MYB88 rabbit polyclonal
Growth protocol Ten-days old plants were grown under 16 h light on 1/2 MS plates
Extracted molecule genomic DNA
Extraction protocol sample was prepared by ChIP using an antibody against FLP/MYB88 proteins
Label biotin
Label protocol The sample was 3'-end-labelled with dd-UTP (Roche) by the terminal transferase (TdT, Roche) reaction for 1.5 h at 37 degrees C.
Hybridization protocol Whole Genome Tiling Array was hybridized for 16 hrs, washed, and stained with a streptavidin-phycoerythrin conjugate on the Affymetrix Fluidics Station 450 according to the standard Affymetrix protocol.
Scan protocol GeneChips were scanned on Affymetrix scanner G7
Description none
Data processing R Bioconductor was used to quantile normalize our ChIP-chip data
Submission date Jan 06, 2010
Last update date Sep 04, 2019
Contact name Zidian Xie
Organization name The Ohio State University
Department Dept. of Plant Cellular and Molecular Biology
Lab 218 Rightmire hall
Street address 1060 Carmack Rd.
City Columbus
State/province OH
ZIP/Postal code 43210
Country USA
Platform ID GPL10977
Series (1)
GSE19763 Genome-wide FLP/MYB88 binding sites in Arabidopsis thaliana
Reanalyzed by GSE136843

Supplementary file Size Download File type/resource
GSM493586.CEL.gz 28.8 Mb (ftp)(http) CEL
GSM493586.txt.gz 16.4 Mb (ftp)(http) TXT
Processed data provided as supplementary file

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