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Sample GSM493586 Query DataSets for GSM493586
Status Public on Sep 01, 2010
Title WF_Grotewold_020907
Sample type genomic
 
Source name FLP/MYB88 ChIP DNA from wild type (Col) shoots (rep 2)
Organism Arabidopsis thaliana
Characteristics age: Ten-day old Green tissue
tissue: Shoots
antibody: FLP/MYB88 rabbit polyclonal
Growth protocol Ten-days old plants were grown under 16 h light on 1/2 MS plates
Extracted molecule genomic DNA
Extraction protocol sample was prepared by ChIP using an antibody against FLP/MYB88 proteins
Label biotin
Label protocol The sample was 3'-end-labelled with dd-UTP (Roche) by the terminal transferase (TdT, Roche) reaction for 1.5 h at 37 degrees C.
 
Hybridization protocol Whole Genome Tiling Array was hybridized for 16 hrs, washed, and stained with a streptavidin-phycoerythrin conjugate on the Affymetrix Fluidics Station 450 according to the standard Affymetrix protocol.
Scan protocol GeneChips were scanned on Affymetrix scanner G7
Description none
Data processing R Bioconductor was used to quantile normalize our ChIP-chip data
 
Submission date Jan 06, 2010
Last update date Sep 04, 2019
Contact name Zidian Xie
E-mail(s) xzd482@yahoo.com
Organization name The Ohio State University
Department Dept. of Plant Cellular and Molecular Biology
Lab 218 Rightmire hall
Street address 1060 Carmack Rd.
City Columbus
State/province OH
ZIP/Postal code 43210
Country USA
 
Platform ID GPL10977
Series (1)
GSE19763 Genome-wide FLP/MYB88 binding sites in Arabidopsis thaliana
Relations
Reanalyzed by GSE136843

Supplementary file Size Download File type/resource
GSM493586.CEL.gz 28.8 Mb (ftp)(http) CEL
GSM493586.txt.gz 16.4 Mb (ftp)(http) TXT
Processed data provided as supplementary file

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