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Sample GSM4946683 Query DataSets for GSM4946683
Status Public on Oct 31, 2021
Title PBMCs infected with IAV and 4OI treated rep2
Sample type RNA
 
Source name Human PBMCs infected with IAV and treated with 4OI for 12 hours
Organism Homo sapiens
Characteristics tissue: Peripheral Blood Mononuclear Cells (primary cells)
treatment: infected with Influenza A virus and treated with 4 Octyl Itaconate (4OI) for 12 hours
Treatment protocol PBMCs were cultured in 6 well plated with/without Influena A virus. Infected cells were either treated with DI, IA or 4OI for 12 hours.
Extracted molecule polyA RNA
Extraction protocol Primary human monocytes were isolated from buffy coats from healthy blood donors by density gradient centrifugation.
mRNA was extracted and purified from PBMCs using MN NucleoSpin RNA kit.
Complementary RNA (cRNA) amplification and coversion to biotinylated double-stranded cDNA (ds-cDNA) was done by Clariom™ S Pico Assay, human Affymetrix protocol from10 ng total RNA (GeneChip® Pico UserGuide 703308 Rev. 4 Affymetrix).
Label biotin
Label protocol Afymetrix protocol
 
Hybridization protocol Hybridization was done for 16 hrs at conditions recommended by the manufacturer. Clariom™ S chips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the Affymetrix GCS 3000. Image analysis was done by Affymetrix® GeneChip® Command Console® Software (AGCC) and Affymetrix® Expression Console™ Software
Description IAV_4OI
Data processing Transcriptome Analysis Console (TAC) software to analyze and visualize global expression patterns of genes, pathways, and network interactions.
 
Submission date Nov 26, 2020
Last update date Oct 31, 2021
Contact name Frank Pessler
E-mail(s) frank.pessler@twincore.de
Phone +49(511)220027167
Organization name Center for Experimental and Clinical Infection Research GmbH
Department Experimental Infection Research
Lab Research Group Biomarkers for Infectious Diseases
Street address Feodor-Lynen-Str. 7-9
City Hannover
ZIP/Postal code 30625
Country Germany
 
Platform ID GPL23159
Series (1)
GSE162210 Expression data from PBMCs infected with influenza A virus and treated with/without Itaconate

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
TC0100006437.hg.1 6.66
TC0100006476.hg.1 8.08
TC0100006479.hg.1 8.7
TC0100006480.hg.1 8.23
TC0100006483.hg.1 8.58
TC0100006486.hg.1 8.72
TC0100006490.hg.1 6.28
TC0100006492.hg.1 7.35
TC0100006494.hg.1 7.05
TC0100006497.hg.1 7.82
TC0100006499.hg.1 8.03
TC0100006501.hg.1 6.72
TC0100006502.hg.1 7.32
TC0100006514.hg.1 8.59
TC0100006516.hg.1 7.55
TC0100006517.hg.1 5.01
TC0100006524.hg.1 8.92
TC0100006540.hg.1 6.22
TC0100006548.hg.1 4.86
TC0100006550.hg.1 6.8

Total number of rows: 21448

Table truncated, full table size 484 Kbytes.




Supplementary file Size Download File type/resource
GSM4946683_Pessler6025_Clariom_S_Human_.CEL.gz 1.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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