|
| Status |
Public on Oct 31, 2021 |
| Title |
PBMCs infected with IAV rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Human PBMCs infected with IAV for 12 hours
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Peripheral Blood Mononuclear Cells (primary cells)
treatment: infected with Influenza A virus for 12 hours
|
| Treatment protocol |
PBMCs were cultured in 6 well plated with/without Influena A virus. Infected cells were either treated with DI, IA or 4OI for 12 hours.
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Primary human monocytes were isolated from buffy coats from healthy blood donors by density gradient centrifugation.
mRNA was extracted and purified from PBMCs using MN NucleoSpin RNA kit.
Complementary RNA (cRNA) amplification and coversion to biotinylated double-stranded cDNA (ds-cDNA) was done by Clariom™ S Pico Assay, human Affymetrix protocol from10 ng total RNA (GeneChip® Pico UserGuide 703308 Rev. 4 Affymetrix).
|
| Label |
biotin
|
| Label protocol |
Afymetrix protocol
|
| |
|
| Hybridization protocol |
Hybridization was done for 16 hrs at conditions recommended by the manufacturer. Clariom™ S chips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GCS 3000. Image analysis was done by Affymetrix® GeneChip® Command Console® Software (AGCC) and Affymetrix® Expression Console™ Software
|
| Description |
IAV
|
| Data processing |
Transcriptome Analysis Console (TAC) software to analyze and visualize global expression patterns of genes, pathways, and network interactions.
|
| |
|
| Submission date |
Nov 26, 2020 |
| Last update date |
Oct 31, 2021 |
| Contact name |
Frank Pessler |
| E-mail(s) |
frank.pessler@twincore.de
|
| Phone |
+49(511)220027167
|
| Organization name |
Center for Experimental and Clinical Infection Research GmbH
|
| Department |
Experimental Infection Research
|
| Lab |
Research Group Biomarkers for Infectious Diseases
|
| Street address |
Feodor-Lynen-Str. 7-9
|
| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE162210 |
Expression data from PBMCs infected with influenza A virus and treated with/without Itaconate |
|
