|
| Status |
Public on Dec 04, 2020 |
| Title |
MV411_10uM_SR0813_R3 |
| Sample type |
SRA |
| |
|
| Source name |
acute myeloid leukemia
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MV4;11
treatment: 10 microM SR-0813
|
| Treatment protocol |
Cells were treated with 1 or 10 µM SR-0813 or DMSO for 4 hours.
|
| Growth protocol |
MV4;11 cells were cultured in RPMI supplemented with 10% FBS, penicillin, and streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with Qiagen Rneasy Mini Kit.
3' mRNA libraries were prepared with Lexogen QuantSeq 3' mRNA-Seq Library Prep Kit FWD for Illumina (Cat. No. 015) according to manufacturer's instructions.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
3' mRNA-seq after 4 hour 10 µM SR0813 treatment
|
| Data processing |
Sequences were aligned and count tables with CPM normaliztion were generated using SLAMdunk (https://t-neumann.github.io/slamdunk/) with the -q option.
Genome_build: hg19
Supplementary_files_format_and_content: Tab-delimited text file matrix with CPM values for each sample.
|
| |
|
| Submission date |
Dec 03, 2020 |
| Last update date |
Dec 04, 2020 |
| Contact name |
Michael Erb |
| E-mail(s) |
michaelerb@scripps.edu
|
| Phone |
858-784-7034
|
| Organization name |
The Scripps Research Institute
|
| Department |
Department of Chemistry
|
| Street address |
10650 N Torrey Pines Rd
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL18573 |
| Series (2) |
| GSE162592 |
Chemical inhibition of ENL/AF9 YEATS domains in acute leukemia [RNA-Seq] |
| GSE162593 |
Chemical inhibition of ENL/AF9 YEATS domains in acute leukemia |
|
| Relations |
| BioSample |
SAMN16988118 |
| SRA |
SRX9624521 |
