|
Status |
Public on Jul 01, 2011 |
Title |
RatSpinalCord-Injury 1 day-5 |
Sample type |
RNA |
|
|
Source name |
spinal cord, injuried, 1 day after surgery
|
Organism |
Rattus norvegicus |
Characteristics |
tissue type: spinal cord gender: female strain: Wistar treatment: Injuried time point: 1 day after surgery
|
Treatment protocol |
Controls: no treatment; Injuried: laminectomy and 200 Kdynes contusion injury at T8 level; Sham: laminectomy at T8 level but no contussion. Contussions using Contusor IH Spinal Cord Impactor (Precision System & Instrumentation, LLC, Va)
|
Extracted molecule |
total RNA |
Extraction protocol |
RNAlater (Qiagen) preservation followed by miRNeasy Mini kit (Qiagen) extraction
|
Label |
Hy3
|
Label protocol |
Using the reactives and protocols included in the miRCURY LNA™ microRNA Power labeling kit, Hy3 dye.
|
|
|
Hybridization protocol |
Following the Exiqon protocols included in the miRCURY LNA™ microRNA Array kit v.11.0
|
Scan protocol |
Arrays were scanned by the Genomic Service of the Madrid Science Park using a GenePix4000B scanner, the GenePix Pro 5.0.1.24 software, and the specific GAL file for the miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno. Scanner settings are detailed in every raw data file.
|
Description |
LS1X367
|
Data processing |
Data provided in the matrix spreadsheet was normalized following the Variance Stabilization Method developed by Huber and developed in R language by the Bioconductor facility.
|
|
|
Submission date |
Jan 14, 2010 |
Last update date |
Jul 01, 2011 |
Contact name |
Rodrigo M Maza |
E-mail(s) |
rodrigom@sescam.jccm.es
|
Organization name |
Hospital Nacional de Parapléjicos (SESCAM)
|
Department |
Unidad de Neurología Experimental
|
Lab |
Molecular Neuroprotection Lab
|
Street address |
Finca La Peraleda s/n
|
City |
Toledo |
State/province |
Toledo |
ZIP/Postal code |
45071 |
Country |
Spain |
|
|
Platform ID |
GPL9908 |
Series (1) |
GSE19890 |
miRNA expression after moderate Spinal Cord Injury in Rats |
|