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Sample GSM497207 Query DataSets for GSM497207
Status Public on Jul 01, 2011
Title RatSpinalCord-Sham 1 day-2
Sample type RNA
 
Source name spinal cord, sham, 1 day after surgery
Organism Rattus norvegicus
Characteristics tissue type: spinal cord
gender: female
strain: Wistar
treatment: Sham
time point: 1 day after surgery
Treatment protocol Controls: no treatment; Injuried: laminectomy and 200 Kdynes contusion injury at T8 level; Sham: laminectomy at T8 level but no contussion. Contussions using Contusor IH Spinal Cord Impactor (Precision System & Instrumentation, LLC, Va)
Extracted molecule total RNA
Extraction protocol RNAlater (Qiagen) preservation followed by miRNeasy Mini kit (Qiagen) extraction
Label Hy3
Label protocol Using the reactives and protocols included in the miRCURY LNA™ microRNA Power labeling kit, Hy3 dye.
 
Hybridization protocol Following the Exiqon protocols included in the miRCURY LNA™ microRNA Array kit v.11.0
Scan protocol Arrays were scanned by the Genomic Service of the Madrid Science Park using a GenePix4000B scanner, the GenePix Pro 5.0.1.24 software, and the specific GAL file for the miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno. Scanner settings are detailed in every raw data file.
Description SH1X882
Data processing Data provided in the matrix spreadsheet was normalized following the Variance Stabilization Method developed by Huber and developed in R language by the Bioconductor facility.
 
Submission date Jan 14, 2010
Last update date Jul 01, 2011
Contact name Rodrigo M Maza
E-mail(s) rodrigom@sescam.jccm.es
Organization name Hospital Nacional de Parapléjicos (SESCAM)
Department Unidad de Neurología Experimental
Lab Molecular Neuroprotection Lab
Street address Finca La Peraleda s/n
City Toledo
State/province Toledo
ZIP/Postal code 45071
Country Spain
 
Platform ID GPL9908
Series (1)
GSE19890 miRNA expression after moderate Spinal Cord Injury in Rats

Data table header descriptions
ID_REF
VALUE VSN transformed expression values

Data table
ID_REF VALUE
1523 6.358
3863 6.358
6203 6.271
8543 6.294
2325 13.091
4665 13.028
7005 13.007
9345 12.866
1389 5.948
3729 5.801
6069 5.975
8409 5.602
234 6.775
2574 6.554
4914 6.759
7254 6.727
2014 6.029
4354 6.055
6694 5.861
9034 5.891

Total number of rows: 7091

Table truncated, full table size 75 Kbytes.




Supplementary file Size Download File type/resource
GSM497207.txt.gz 183.7 Kb (ftp)(http) TXT
Processed data included within Sample table

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