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Sample GSM497228 Query DataSets for GSM497228
Status Public on Jul 01, 2011
Title RatSpinalCord-Sham 7 day-3
Sample type RNA
 
Source name spinal cord, sham, 7 days after surgery
Organism Rattus norvegicus
Characteristics tissue type: spinal cord
gender: female
strain: Wistar
treatment: Sham
time point: 7 days after surgery
Treatment protocol Controls: no treatment; Injuried: laminectomy and 200 Kdynes contusion injury at T8 level; Sham: laminectomy at T8 level but no contussion. Contussions using Contusor IH Spinal Cord Impactor (Precision System & Instrumentation, LLC, Va)
Extracted molecule total RNA
Extraction protocol RNAlater (Qiagen) preservation followed by miRNeasy Mini kit (Qiagen) extraction
Label Hy3
Label protocol Using the reactives and protocols included in the miRCURY LNA™ microRNA Power labeling kit, Hy3 dye.
 
Hybridization protocol Following the Exiqon protocols included in the miRCURY LNA™ microRNA Array kit v.11.0
Scan protocol Arrays were scanned by the Genomic Service of the Madrid Science Park using a GenePix4000B scanner, the GenePix Pro 5.0.1.24 software, and the specific GAL file for the miRCURY LNA microRNA Array, v.11.0 - hsa, mmu & rno. Scanner settings are detailed in every raw data file.
Description SH7X211
Data processing Data provided in the matrix spreadsheet was normalized following the Variance Stabilization Method developed by Huber and developed in R language by the Bioconductor facility.
 
Submission date Jan 14, 2010
Last update date Jul 01, 2011
Contact name Rodrigo M Maza
E-mail(s) rodrigom@sescam.jccm.es
Organization name Hospital Nacional de Parapléjicos (SESCAM)
Department Unidad de Neurología Experimental
Lab Molecular Neuroprotection Lab
Street address Finca La Peraleda s/n
City Toledo
State/province Toledo
ZIP/Postal code 45071
Country Spain
 
Platform ID GPL9908
Series (1)
GSE19890 miRNA expression after moderate Spinal Cord Injury in Rats

Data table header descriptions
ID_REF
VALUE VSN transformed expression values

Data table
ID_REF VALUE
1523 6.395
3863 6.213
6203 6.241
8543 6.320
2325 11.915
4665 11.884
7005 11.725
9345 11.558
1389 6.489
3729 5.907
6069 5.801
8409 5.974
234 6.720
2574 6.534
4914 6.534
7254 6.777
2014 6.006
4354 5.974
6694 6.186
9034 6.157

Total number of rows: 7091

Table truncated, full table size 75 Kbytes.




Supplementary file Size Download File type/resource
GSM497228.txt.gz 180.4 Kb (ftp)(http) TXT
Processed data included within Sample table

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