|
| Status |
Public on Feb 10, 2021 |
| Title |
scRNA_seq 2 |
| Sample type |
SRA |
| |
|
| Source name |
P21 Brain
|
| Organism |
Mus musculus |
| Characteristics |
strain: C56BL/6j
genotype: Sox10:Cre RCE+
|
| Growth protocol |
Brain was dissociated using Miltenyi neural tissue dissociation kit P (130-092-628), cleared with Debris removal solution (130-109-398), filtered through 50 um strainer and FACS sorted for GFP+/GFP- on FACS Aria III or FACS Aria Fusion
|
| Extracted molecule |
total RNA |
| Extraction protocol |
scRNA-seq library was constructed using 10x chromium 3' gene expression kit v3
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Data processing |
Fastq files were processed using cellranger 3.0.2
Only cells with GFP reads were considered
h5 matrices from cellranger are provided as
Genome_build: mm10
Supplementary_files_format_and_content: raw h5 matrices with counts; csv with cells metadata after filtering
|
| |
|
| Submission date |
Dec 18, 2020 |
| Last update date |
Feb 11, 2021 |
| Contact name |
Marek Bartosovic |
| E-mail(s) |
bartosovic.marek@gmail.com
|
| Organization name |
Karolinska Institute
|
| Lab |
Goncalo Castelo-Branco
|
| Street address |
Solnavagen 9
|
| City |
Stockholm |
| ZIP/Postal code |
12432 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE163484 |
Single cell RNA-seq of Sox10-Cre::GFP+ population |
| GSE163532 |
Single cell profiling of histone modifications and transcription factors in the mouse brain |
|
| Relations |
| BioSample |
SAMN17112671 |
| SRA |
SRX9702232 |
