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Status |
Public on Jun 30, 2021 |
Title |
98NFA86 |
Sample type |
RNA |
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|
Source name |
C01P007
|
Organism |
Homo sapiens |
Characteristics |
diagnosis: ALS tissue: peripheral blood treatment: Placebo time point: Day1
|
Extracted molecule |
total RNA |
Extraction protocol |
White blood cell isolation: White blood cells were isolated using LeukoLOCK TM filters Total RNA extraction: Total RNA was isolated using the LeukoLOCK TM Total RNA isolation kit (AmbionTM) as per manufacturer's instructions.
|
Label |
Biotin
|
Label protocol |
200ng of total RNA was used to produce antisense RNA (cRNA) and cDNA which was then fragmented and biotinylated using Affymetrix GeneChip® WT PLUS Kit according to manufacturer's protocol.
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|
|
Hybridization protocol |
Fragmented and labelled ss cDNA was hybridized on Human Clariom D Affymetrix® chips.
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Scan protocol |
The GeneChips were scanned in the Affymetrix GCS3000 scanner and the resultant image processed using the AGCC software package to prepare the .CEL files
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Description |
Gene expression data from human peripheral blood
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Data processing |
Gene expression data was normalised using the signal space transformation robust multi-chip analysis (SST-RMA) method. Data was analysed using TAC and Limma software.
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|
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Submission date |
Dec 20, 2020 |
Last update date |
Jun 30, 2021 |
Contact name |
Ilaria Giovannelli |
E-mail(s) |
igiovannelli1@sheffield.ac.uk
|
Organization name |
University of Sheffield
|
Department |
Sheffield Institute for Translational Neuroscience
|
Street address |
385a Glossop Road
|
City |
Sheffield |
ZIP/Postal code |
S10 2HQ |
Country |
United Kingdom |
|
|
Platform ID |
GPL23126 |
Series (1) |
GSE163560 |
Amyotrophic Lateral Sclerosis Transcriptomics Reveals Immunological Effects of Low-Dose Interleukin-2. |
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