|
Status |
Public on Dec 06, 2021 |
Title |
B0: B cell population at day 0 scRNA-seq |
Sample type |
SRA |
|
|
Source name |
B cell population at day 0
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6J gender: Female cell type: day 0 colon derived B cells treatment: DSS treatment
|
Growth protocol |
B cells were isolated from the colon of mice on day 0 (no treatment) or day 14 (recovery) after 7 days of DSS treatment by flow cytometric sorting and analyzed by 10X sequencing
|
Extracted molecule |
total RNA |
Extraction protocol |
cells were processed according to 10x Genomics protocol for Chromium Single cell 3’ kit (version 2)
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Data processing |
Base calling and preprocessing using cell ranger alignment and count using cell ranger pipline seurat 3.0 pipeline for downstream analysis Genome_build: mm10
|
|
|
Submission date |
Dec 21, 2020 |
Last update date |
Dec 06, 2021 |
Contact name |
Eduardo J Villablanca |
E-mail(s) |
eduardo.villablanca@ki.se
|
Organization name |
Karolinska Institute
|
Department |
CMM
|
Street address |
visiongatan 18
|
City |
Stockholm |
ZIP/Postal code |
17176 |
Country |
Sweden |
|
|
Platform ID |
GPL17021 |
Series (1) |
GSE163637 |
10X-single cell seq of B cells during colitis (d0 and d14) |
|
Relations |
BioSample |
SAMN17129524 |
SRA |
SRX9711360 |