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Sample GSM4983264 Query DataSets for GSM4983264
Status Public on Dec 06, 2021
Title B14: B cell population at day 14 scRNA-seq
Sample type SRA
Source name B cell population at day 14
Organism Mus musculus
Characteristics strain: C57BL/6J
gender: Female
cell type: day 14 colon derived B cells
treatment: DSS treatment
Growth protocol B cells were isolated from the colon of mice on day 0 (no treatment) or day 14 (recovery) after 7 days of DSS treatment by flow cytometric sorting and analyzed by 10X sequencing
Extracted molecule total RNA
Extraction protocol cells were processed according to 10x Genomics protocol for Chromium Single cell 3’ kit (version 2)
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
Data processing Base calling and preprocessing using cell ranger
alignment and count using cell ranger pipline
seurat 3.0 pipeline for downstream analysis
Genome_build: mm10
Submission date Dec 21, 2020
Last update date Dec 06, 2021
Contact name Eduardo J Villablanca
Organization name Karolinska Institute
Department CMM
Street address visiongatan 18
City Stockholm
ZIP/Postal code 17176
Country Sweden
Platform ID GPL17021
Series (1)
GSE163637 10X-single cell seq of B cells during colitis (d0 and d14)
BioSample SAMN17129523
SRA SRX9711361

Supplementary file Size Download File type/resource
GSM4983264_B14_barcodes.tsv.gz 24.0 Kb (ftp)(http) TSV
GSM4983264_B14_features.tsv.gz 272.8 Kb (ftp)(http) TSV
GSM4983264_B14_lognorm_count.txt.gz 35.2 Mb (ftp)(http) TXT
GSM4983264_B14_matrix.mtx.gz 16.4 Mb (ftp)(http) MTX
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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