|
| Status |
Public on Jul 06, 2022 |
| Title |
MSH6-1mM |
| Sample type |
SRA |
| |
|
| Source name |
Hela
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: treat with 1mM H2O2 for 30min
cell type: Hela cell
chip antibody: MSH6 (BD, 610919)
|
| Treatment protocol |
Cells were treated with 1mM H2O2 for 30min.
|
| Growth protocol |
Hela cells were cultured on uncoated plate in DMEM medium with 10% fetal bovine serum.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Histone-DNA complexes were isolated with antibody from sonicated whole cell lysate.
Libraries were prepared according to NEBNext Ultra II DNA Library Prep kit.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
alignment: bowtie2
peak calling: MACS
Genome_build: hg19
Supplementary_files_format_and_content: bedgraph or bed files for the output by MACS
|
| |
|
| Submission date |
Dec 28, 2020 |
| Last update date |
Jul 06, 2022 |
| Contact name |
jinlong lu |
| E-mail(s) |
bioyuyang@hotmail.com
|
| Phone |
5853098469
|
| Organization name |
University of Rochester
|
| Lab |
Gorbunova & Seluanov Labs
|
| Street address |
213 Hutchinson Hall
|
| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14627 |
| Country |
USA |
| |
|
| Platform ID |
GPL20795 |
| Series (1) |
| GSE163940 |
Mismatch repair protein coordinate with SETD2 to activate DNA oxidative damage response and repair in human cells |
|
| Relations |
| BioSample |
SAMN17171984 |
| SRA |
SRX9742022 |
