|
| Status |
Public on Jul 07, 2010 |
| Title |
ETV6/RUNX1-positive acute lymphoblastic leukemia, patient sample 15 (Nsp) |
| Sample type |
genomic |
| |
|
| Source name |
ETV6/RUNX1-positive acute lymphoblastic leukemia, patient sample 15
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Acute lymphoblastic leukemia
|
| Growth protocol |
The cell line was grown according to standard conditions.
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA was extracted from leukemic blasts obtained from bone marrow at diagnosis using standard methods. Genomic DNA from the REH cell line was extracted from cultured cells using standard methods. DNA quality and quantity was assessed using a Nanodrop Spectrophotometer.
|
| Label |
Biotin
|
| Label protocol |
The protocol provided by the manufacturer (Affymetrix) was used
|
| |
|
| Hybridization protocol |
The protocol provided by the manufacturer (Affymetrix) was used
|
| Scan protocol |
The chips were washed and stained according to the manufacturer's instructions and scanned using a GeneChip Scanner 3000
|
| Description |
Hybridized to 250K_Nsp
|
| Data processing |
CEL files containing raw signal intensities were exported from the GeneChip Operating Software. The CEL files were imported into GeneChip Genotyping Analysis Software to produce genotype calls using the BRLMM algorithm (CHP-files).
Signal: Summarized signal; Call = Genotype Call (SNP call): AA, AB, BB, and NoCall
|
| |
|
| Submission date |
Jan 21, 2010 |
| Last update date |
Jul 07, 2010 |
| Contact name |
Henrik Lilljebjörn |
| Organization name |
Lund University
|
| Department |
Dept of Clinical Genetics
|
| Street address |
Lund University Hospital
|
| City |
Lund |
| ZIP/Postal code |
22185 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL3718 |
| Series (1) |
| GSE19996 |
Affy SNP-array data for 23 ETV6/RUNX1-positive ALL patient samples and one cell line |
|
