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Status |
Public on Mar 09, 2021 |
Title |
scRNA-seq of a mixture of 16 celltypes from 7 species |
Sample type |
SRA |
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Source name |
HEK-293T, RPE-1, Jurkat, K-562, HCT116, HepG2, Hela, 786-0, RAW264.7, CT26, 4T1, REF, MDCK, CHO, Vero, S2
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Organisms |
Drosophila melanogaster; Homo sapiens; Canis lupus familiaris; Cricetulus griseus; Mus musculus; Rattus norvegicus; Chlorocebus sabaeus |
Characteristics |
cell line: mixture (HEK-293T, RPE-1, Jurkat, K-562, HCT116, HepG2, Hela, 786-0, RAW264.7, CT26, 4T1, REF, MDCK, CHO, Vero, S2)
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Extracted molecule |
polyA RNA |
Extraction protocol |
Single cells were lysed in droplets using the 10X Chromium instrument. Reverse transcription was performed in droplet and library construction was performed according to the 10X Chromium Single Cell 3' Reagent Kit User Guide Library preparation was performed according to the 10X Chromium Single Cell 3' Reagent Kit User Guide and using a custom CASB library preparation protocol.
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
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Data processing |
Gene-cell matrices were determined using 10X Genomics Cellranger version 3.1.0 with default parameters (cellranger count). Genome_build: concatenated genome, ensembl version 102 of 7 species (Homo sapiens, Mus musculus, Rattus norvegicus, Canis familiaris, Cricetulus griseus, Chlorocebus_sabaeus, Drosophila melanogaster) Supplementary_files_format_and_content: h5 file produced by cellranger
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Submission date |
Jan 18, 2021 |
Last update date |
Mar 09, 2021 |
Contact name |
Guipeng Li |
E-mail(s) |
guipeng.lee@gmail.com, ligp@sustech.edu.cn
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Organization name |
Southern University of Science and Technology
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Street address |
1088 Xueyuan Avenue
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City |
Shenzhen |
ZIP/Postal code |
518055 |
Country |
China |
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Platform ID |
GPL29623 |
Series (2) |
GSE153116 |
CASB: A concanavalin A-based sample barcoding strategy for single-cell sequencing |
GSE165047 |
CASB: A concanavalin A-based sample barcoding strategy for single-cell sequencing [scRNA-seq] |
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Relations |
BioSample |
SAMN17376018 |
SRA |
SRX9892150 |
Supplementary data files not provided |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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