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Sample GSM5025077 Query DataSets for GSM5025077
Status Public on Mar 09, 2021
Title CASB library for scRNA-seq of a mixture of 16 celltypes from 7 species
Sample type SRA
 
Source name HEK-293T, RPE-1, Jurkat, K-562, HCT116, HepG2, Hela, 786-0, RAW264.7, CT26, 4T1, REF, MDCK, CHO, Vero, S2
Organisms Drosophila melanogaster; Homo sapiens; Canis lupus familiaris; Cricetulus griseus; Mus musculus; Rattus norvegicus; Chlorocebus sabaeus
Characteristics cell line: mixture (HEK-293T, RPE-1, Jurkat, K-562, HCT116, HepG2, Hela, 786-0, RAW264.7, CT26, 4T1, REF, MDCK, CHO, Vero, S2)
Extracted molecule polyA RNA
Extraction protocol Single cells were lysed in droplets using the 10X Chromium instrument. Reverse transcription was performed in droplet and library construction was performed according to the 10X Chromium Single Cell 3' Reagent Kit User Guide
Library preparation was performed according to the 10X Chromium Single Cell 3' Reagent Kit User Guide and using a custom CASB library preparation protocol.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing Gene-cell matrices were determined using 10X Genomics Cellranger version 3.1.0 with default parameters (cellranger count).
Genome_build: concatenated genome, ensembl version 102 of 7 species (Homo sapiens, Mus musculus, Rattus norvegicus, Canis familiaris, Cricetulus griseus, Chlorocebus_sabaeus, Drosophila melanogaster)
Supplementary_files_format_and_content: h5 file produced by cellranger
 
Submission date Jan 18, 2021
Last update date Mar 09, 2021
Contact name Guipeng Li
E-mail(s) guipeng.lee@gmail.com, ligp@sustech.edu.cn
Organization name Southern University of Science and Technology
Street address 1088 Xueyuan Avenue
City Shenzhen
ZIP/Postal code 518055
Country China
 
Platform ID GPL29623
Series (2)
GSE153116 CASB: A concanavalin A-based sample barcoding strategy for single-cell sequencing
GSE165047 CASB: A concanavalin A-based sample barcoding strategy for single-cell sequencing [scRNA-seq]
Relations
BioSample SAMN17376017
SRA SRX9892151

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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