|
| Status |
Public on Apr 23, 2021 |
| Title |
MET-3 |
| Sample type |
RNA |
| |
|
| Source name |
Hepatic progenitor cells
|
| Organism |
Rattus norvegicus |
| Characteristics |
cell type: In vitro cultured hepatic progenitor cells
strain: SD Rat
|
| Treatment protocol |
Hepatic progenitor cells were treated with 1ng/ml TGF-b1 in 10%FBS DMEM/F12 for 16 days to induce epithelial-mesenchymal transition. The 16-day TGF-b1-treated cells were cultured in 10%FBS DMEM/F12 with 1ng/ml EGF and 0.5ng/ml SCF for 7 days to induce mesenchymal-epithelial transition.
|
| Growth protocol |
Hepatic progenitor cells were cultured in 10%FBS DMEM/F12 with 1ng/ml EGF and 0.5ng/ml SCF.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated by TRIzol Reagent and RNA Neasy Mini Kit.
Target prepared by GeneChip WT PLUS Reagent kit.
|
| Label |
biotin
|
| Label protocol |
standard Affymentrix protocol
|
| |
|
| Hybridization protocol |
standard Affymentrix protocol
|
| Scan protocol |
standard Affymentrix protocol
|
| Data processing |
Affymetrix® Expression Console™ software (v.1.0)
Partek Genomics Suite 6.6
RMA normalization
Histogram analysis and PCA analysis
Differentially Expressed Genes Between Groups
raw CEL data for each gene and each sample
|
| |
|
| Submission date |
Jan 31, 2021 |
| Last update date |
Apr 23, 2021 |
| Contact name |
Ping Wang |
| E-mail(s) |
wangping2009@ccmu.edu.cn
|
| Organization name |
Beijing Friendship Hospital
|
| Street address |
Yong An Road No.95
|
| City |
Beijing |
| ZIP/Postal code |
100050 |
| Country |
China |
| |
|
| Platform ID |
GPL22388 |
| Series (1) |
| GSE165858 |
Gene Transcriptional Variation of Hepatic Progenitor Cells after TGF-𝛽1-Induced EMT Transition and EGF-Induced MET Reversion |
|
