|
| Status |
Public on Feb 04, 2021 |
| Title |
SD_Mut |
| Sample type |
RNA |
| |
|
| Source name |
hd1-3 mutant rice leaves,short day conditions
|
| Organism |
Oryza sativa |
| Characteristics |
growth conditions: short day
tissue: leaves
genotype: hd1-3 mutant
|
| Growth protocol |
5-day-old seedlings of hd1-3 mutant and the wild-type Zhonghua11 after germination were grown in artificial climate chamber for 30 days. The growth conditions were set as 11.5 h light/ 12.5 h dark, 28℃ for short-day conditions and 15 h light/ 9 h dark, 28℃ for long-day conditions, respectively.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Leaves of 35-day-old rice plants were collected. For short-day conditions, the samples were collected at 3 h after light; for long-day conditions, the samples were collected at 5 h after light. Total RNAs were extracted using TRIzol method and mRNAs were purified using mRNA purification kit.
|
| Label |
biotin
|
| Label protocol |
Biotinylated RNA were prepared according to the standard Affymetrix protocol.
|
| |
|
| Hybridization protocol |
Affymetrix gene chip hybridization, washing, staining, and scanning were carried out according to manufacturer's instructions.
|
| Scan protocol |
GeneChips were scanned using Affymetrix Scanner 3000 with default settings.
|
| Data processing |
The microarray data were analyzed using MAS5 algorithm.
|
| |
|
| Submission date |
Feb 03, 2021 |
| Last update date |
Feb 04, 2021 |
| Contact name |
Xuan Ma |
| E-mail(s) |
skyxma@tjnu.edu.cn
|
| Organization name |
Tianjin Normal University
|
| Department |
College of Life Sciences
|
| Street address |
Binshui West Road
|
| City |
Tianjin |
| ZIP/Postal code |
300387 |
| Country |
China |
| |
|
| Platform ID |
GPL2025 |
| Series (1) |
| GSE166053 |
Microarray comparative analysis of wild-type and hd1-3 mutant rice |
|
