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Status |
Public on Feb 04, 2021 |
Title |
LD_WT |
Sample type |
RNA |
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|
Source name |
wild-type rice leaves,long day conditions
|
Organism |
Oryza sativa |
Characteristics |
growth conditions: long day tissue: leaves genotype: wild type Zhonghua11
|
Growth protocol |
5-day-old seedlings of hd1-3 mutant and the wild-type Zhonghua11 after germination were grown in artificial climate chamber for 30 days. The growth conditions were set as 11.5 h light/ 12.5 h dark, 28℃ for short-day conditions and 15 h light/ 9 h dark, 28℃ for long-day conditions, respectively.
|
Extracted molecule |
total RNA |
Extraction protocol |
Leaves of 35-day-old rice plants were collected. For short-day conditions, the samples were collected at 3 h after light; for long-day conditions, the samples were collected at 5 h after light. Total RNAs were extracted using TRIzol method and mRNAs were purified using mRNA purification kit.
|
Label |
biotin
|
Label protocol |
Biotinylated RNA were prepared according to the standard Affymetrix protocol.
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|
|
Hybridization protocol |
Affymetrix gene chip hybridization, washing, staining, and scanning were carried out according to manufacturer's instructions.
|
Scan protocol |
GeneChips were scanned using Affymetrix Scanner 3000 with default settings.
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Data processing |
The microarray data were analyzed using MAS5 algorithm.
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Submission date |
Feb 03, 2021 |
Last update date |
Feb 04, 2021 |
Contact name |
Xuan Ma |
E-mail(s) |
skyxma@tjnu.edu.cn
|
Organization name |
Tianjin Normal University
|
Department |
College of Life Sciences
|
Street address |
Binshui West Road
|
City |
Tianjin |
ZIP/Postal code |
300387 |
Country |
China |
|
|
Platform ID |
GPL2025 |
Series (1) |
GSE166053 |
Microarray comparative analysis of wild-type and hd1-3 mutant rice |
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