|
Status |
Public on Feb 04, 2021 |
Title |
CD62L-low CMP rep-3 |
Sample type |
SRA |
|
|
Source name |
murine hematopoietic progenitor cells
|
Organism |
Mus musculus |
Characteristics |
cell type: hematopoietic progenitors from bone marrow strain: C57/B6 cell subset: CD62L-low CMP
|
Extracted molecule |
total RNA |
Extraction protocol |
Mononuclear cells were extracted from bone marrow cells, then each subset was sorted by flow cytometry and RNA was extracted. RNA libraries were prepared for sequencing using SMARTer Stranded RNA-Seq Kit.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
HiSeq X Ten |
|
|
Data processing |
Base calling was performed by Illumina RTA software in sequencer, further demultiplexing was performed by Illumina bcl2fastq software. Adaptor trimming, mapping and acquiring read count data were performed by Trim-Galore, Hisat2 and Htseq. bulk CMP rep-2 was removed from analysis due to low mapping rate. Genome_build: mm10 Supplementary_files_format_and_content: Matrix table with raw read counts for every gene and every sample
|
|
|
Submission date |
Feb 03, 2021 |
Last update date |
Feb 04, 2021 |
Contact name |
Yusuke Ito |
E-mail(s) |
yuusuitou-tky@umin.ac.jp
|
Phone |
+81333531211
|
Organization name |
Keio University
|
Department |
Division of Tumor Immunology
|
Street address |
35 Shinanomachi
|
City |
Shinjuku-ku |
State/province |
Tokyo |
ZIP/Postal code |
1608582 |
Country |
Japan |
|
|
Platform ID |
GPL21273 |
Series (1) |
GSE166065 |
CD62L expression level determines the cell fate of myeloid progenitors in mice and humans |
|
Relations |
BioSample |
SAMN17767475 |
SRA |
SRX10011505 |