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Sample GSM5061100 Query DataSets for GSM5061100
Status Public on Feb 04, 2021
Title CD62L-high CMP rep-2
Sample type SRA
 
Source name murine hematopoietic progenitor cells
Organism Mus musculus
Characteristics cell type: hematopoietic progenitors from bone marrow
strain: C57/B6
cell subset: CD62L-high CMP
Extracted molecule total RNA
Extraction protocol Mononuclear cells were extracted from bone marrow cells, then each subset was sorted by flow cytometry and RNA was extracted.
RNA libraries were prepared for sequencing using SMARTer Stranded RNA-Seq Kit.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
 
Data processing Base calling was performed by Illumina RTA software in sequencer, further demultiplexing was performed by Illumina bcl2fastq software.
Adaptor trimming, mapping and acquiring read count data were performed by Trim-Galore, Hisat2 and Htseq.
bulk CMP rep-2 was removed from analysis due to low mapping rate.
Genome_build: mm10
Supplementary_files_format_and_content: Matrix table with raw read counts for every gene and every sample
 
Submission date Feb 03, 2021
Last update date Feb 04, 2021
Contact name Yusuke Ito
E-mail(s) yuusuitou-tky@umin.ac.jp
Phone +81333531211
Organization name Keio University
Department Division of Tumor Immunology
Street address 35 Shinanomachi
City Shinjuku-ku
State/province Tokyo
ZIP/Postal code 1608582
Country Japan
 
Platform ID GPL21273
Series (1)
GSE166065 CD62L expression level determines the cell fate of myeloid progenitors in mice and humans
Relations
BioSample SAMN17767472
SRA SRX10011507

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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