|
| Status |
Public on Feb 04, 2021 |
| Title |
rat-METH-METH challenge-1 rep |
| Sample type |
RNA |
| |
|
| Source name |
total mRNA from PFC METH-METH group
|
| Organism |
Rattus norvegicus |
| Characteristics |
strain: Sprague-Dawley
tissue: prefrontal cortex
gender: male
|
| Treatment protocol |
The rats were administrated with METH (5 mg/kg, s.c.) or normal saline (NS) (1 ml/kg, s.c.) once daily in acute phase or for 7 days in development phase. On d8-14, the rats were housed in home cages, and nothing was injected in withdrawal phase. On d15, METH (1 mg/kg, s.c.) was used to challenge in the rats that were administrated with NS or METH in development phase, and then the locomotor activity was recorded for 60 min in challenge phase.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The fluorescently amplified RNA targets were prepared from 2.5 μg of total RNA samples using the OneArray® Amino Allyl aRNA Amplification Kit (Phalanx Biotech Group)。
|
| Label |
Cy5
|
| Label protocol |
the amplified RNA argets were labeled with dye (Amersham Pharmacia, Piscataway, NJ, USA).
|
| |
|
| Hybridization protocol |
the labeled RNA targets were hybridized to the Rat Whole Genome OneArray® (ROA 1.1) (Phalanx Biotech Group) according to the Phalanx hybridization protocol.
|
| Scan protocol |
The slides were scanned using an Axon 4000 scanner (Molecular Devices, Sunnyvale, CA, USA).
|
| Data processing |
The Cy5 fluorescence intensity of each spot was analyzed using GenePix pro software (Molecular Devices).
The signal intensity of each spot was loaded into the Rosetta Resolver System® (Rosetta Biosoftware) for data analysis.
|
| |
|
| Submission date |
Feb 03, 2021 |
| Last update date |
Feb 04, 2021 |
| Contact name |
Hui Li |
| E-mail(s) |
lihuiwzsszx@163.com
|
| Organization name |
Beijing Key Laboratory of Neuropsychopharmacology
|
| Street address |
27th Taiping Road
|
| City |
Beijing |
| ZIP/Postal code |
100850 |
| Country |
China |
| |
|
| Platform ID |
GPL13694 |
| Series (2) |
| GSE166094 |
Behavioral Sensitization Induced by Methamphetamine Causes Differential Alterations in Gene Expression and Histone Acetylation of the Prefrontal Cortex in Rats (mRNA dataset) |
| GSE166096 |
Behavioral Sensitization Induced by Methamphetamine Causes Differential Alterations in Gene Expression and Histone Acetylation of the Prefrontal Cortex in Rats |
|
