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Sample GSM5061760 Query DataSets for GSM5061760
Status Public on Feb 04, 2021
Title rat-NS-METH challenge-1 rep
Sample type RNA
 
Source name total mRNA from PFC METH-NS group
Organism Rattus norvegicus
Characteristics strain: Sprague-Dawley
tissue: prefrontal cortex
gender: male
Treatment protocol The rats were administrated with METH (5 mg/kg, s.c.) or normal saline (NS) (1 ml/kg, s.c.) once daily in acute phase or for 7 days in development phase. On d8-14, the rats were housed in home cages, and nothing was injected in withdrawal phase. On d15, METH (1 mg/kg, s.c.) was used to challenge in the rats that were administrated with NS or METH in development phase, and then the locomotor activity was recorded for 60 min in challenge phase.
Extracted molecule total RNA
Extraction protocol The fluorescently amplified RNA targets were prepared from 2.5 μg of total RNA samples using the OneArray® Amino Allyl aRNA Amplification Kit (Phalanx Biotech Group)。
Label Cy5
Label protocol the amplified RNA argets were labeled with dye (Amersham Pharmacia, Piscataway, NJ, USA).
 
Hybridization protocol the labeled RNA targets were hybridized to the Rat Whole Genome OneArray® (ROA 1.1) (Phalanx Biotech Group) according to the Phalanx hybridization protocol.
Scan protocol The slides were scanned using an Axon 4000 scanner (Molecular Devices, Sunnyvale, CA, USA).
Data processing The Cy5 fluorescence intensity of each spot was analyzed using GenePix pro software (Molecular Devices).
The signal intensity of each spot was loaded into the Rosetta Resolver System® (Rosetta Biosoftware) for data analysis.
 
Submission date Feb 03, 2021
Last update date Feb 04, 2021
Contact name Hui Li
E-mail(s) lihuiwzsszx@163.com
Organization name Beijing Key Laboratory of Neuropsychopharmacology
Street address 27th Taiping Road
City Beijing
ZIP/Postal code 100850
Country China
 
Platform ID GPL13694
Series (2)
GSE166094 Behavioral Sensitization Induced by Methamphetamine Causes Differential Alterations in Gene Expression and Histone Acetylation of the Prefrontal Cortex in Rats (mRNA dataset)
GSE166096 Behavioral Sensitization Induced by Methamphetamine Causes Differential Alterations in Gene Expression and Histone Acetylation of the Prefrontal Cortex in Rats

Data table header descriptions
ID_REF
VALUE Cy5 signal intensity

Data table
ID_REF VALUE
PH_rn_0000001 658.098694
PH_rn_0000002 174.687302
PH_rn_0000003 12.085285
PH_rn_0000004 145.023422
PH_rn_0000005 9.88796
PH_rn_0000006 1350.25586
PH_rn_0000007 10.986623
PH_rn_0000008 127.444824
PH_rn_0000009 1170.07532
PH_rn_0000010 926.172302
PH_rn_0000011 352.670593
PH_rn_0000012 2923.54028
PH_rn_0000013 694.354553
PH_rn_0000014 1616.1322
PH_rn_0000015 1301.91479
PH_rn_0000016 38.453178
PH_rn_0000017 530.65387
PH_rn_0000018 43.946491
PH_rn_0000019 37.354515
PH_rn_0000020 9.88796

Total number of rows: 24358

Table truncated, full table size 580 Kbytes.




Supplementary file Size Download File type/resource
GSM5061760_H003-1100508811.gpr.gz 2.5 Mb (ftp)(http) GPR
Processed data included within Sample table

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