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Sample GSM5061761 Query DataSets for GSM5061761
Status Public on Feb 04, 2021
Title rat-NS-NS challenge-1 rep
Sample type RNA
 
Source name total mRNA from PFC NS-NS group
Organism Rattus norvegicus
Characteristics strain: Sprague-Dawley
tissue: prefrontal cortex
gender: male
Treatment protocol The rats were administrated with METH (5 mg/kg, s.c.) or normal saline (NS) (1 ml/kg, s.c.) once daily in acute phase or for 7 days in development phase. On d8-14, the rats were housed in home cages, and nothing was injected in withdrawal phase. On d15, METH (1 mg/kg, s.c.) was used to challenge in the rats that were administrated with NS or METH in development phase, and then the locomotor activity was recorded for 60 min in challenge phase.
Extracted molecule total RNA
Extraction protocol The fluorescently amplified RNA targets were prepared from 2.5 μg of total RNA samples using the OneArray® Amino Allyl aRNA Amplification Kit (Phalanx Biotech Group)。
Label Cy5
Label protocol the amplified RNA argets were labeled with dye (Amersham Pharmacia, Piscataway, NJ, USA).
 
Hybridization protocol the labeled RNA targets were hybridized to the Rat Whole Genome OneArray® (ROA 1.1) (Phalanx Biotech Group) according to the Phalanx hybridization protocol.
Scan protocol The slides were scanned using an Axon 4000 scanner (Molecular Devices, Sunnyvale, CA, USA).
Data processing The Cy5 fluorescence intensity of each spot was analyzed using GenePix pro software (Molecular Devices).
The signal intensity of each spot was loaded into the Rosetta Resolver System® (Rosetta Biosoftware) for data analysis.
 
Submission date Feb 03, 2021
Last update date Feb 04, 2021
Contact name Hui Li
E-mail(s) lihuiwzsszx@163.com
Organization name Beijing Key Laboratory of Neuropsychopharmacology
Street address 27th Taiping Road
City Beijing
ZIP/Postal code 100850
Country China
 
Platform ID GPL13694
Series (2)
GSE166094 Behavioral Sensitization Induced by Methamphetamine Causes Differential Alterations in Gene Expression and Histone Acetylation of the Prefrontal Cortex in Rats (mRNA dataset)
GSE166096 Behavioral Sensitization Induced by Methamphetamine Causes Differential Alterations in Gene Expression and Histone Acetylation of the Prefrontal Cortex in Rats

Data table header descriptions
ID_REF
VALUE Cy5 signal intensity

Data table
ID_REF VALUE
PH_rn_0000001 625.472107
PH_rn_0000002 352.908661
PH_rn_0000003 11.187306
PH_rn_0000004 138.315796
PH_rn_0000005 7.119195
PH_rn_0000006 1234.67188
PH_rn_0000007 27.459751
PH_rn_0000008 133.230652
PH_rn_0000009 977.36377
PH_rn_0000010 1082.11768
PH_rn_0000011 314.261597
PH_rn_0000012 2605.62524
PH_rn_0000013 989.568115
PH_rn_0000014 1912.01233
PH_rn_0000015 1298.74463
PH_rn_0000016 32.544891
PH_rn_0000017 693.612976
PH_rn_0000018 27.459751
PH_rn_0000019 36.613003
PH_rn_0000020 7.119195

Total number of rows: 24358

Table truncated, full table size 581 Kbytes.




Supplementary file Size Download File type/resource
GSM5061761_H004-1100508815.gpr.gz 2.5 Mb (ftp)(http) GPR
Processed data included within Sample table

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