|
| Status |
Public on Apr 27, 2021 |
| Title |
rfl8-3HA_(2) |
| Sample type |
SRA |
| |
|
| Source name |
rfl8-3HA_2
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
genotype: wild type (rfl8 rfl8 CDS fused in frame with 3-HA tag)
growth: planting-date=12-FEB-19,harvest-date=29-MAR-19
tissue: flower
|
| Treatment protocol |
RNAseI-genetic treatment-enzymatic,earthworms-rnaseqi RNaseI treatment of mitochondrial extracts
|
| Growth protocol |
Plants were grown in a greenhouse, in soil, in long day condition
|
| Extracted molecule |
total RNA |
| Extraction protocol |
rfl8-3HA_2(2mg)
rna_selection:ribosomal depletion, single, stranded:no, Protocol:5132-05-NEXTflex-Small-RNA-Seq-v3-16-06.pdf
|
| |
|
| Library strategy |
RIP-Seq |
| Library source |
transcriptomic |
| Library selection |
other |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Genome_build: genome of Arabidopsis mitochondrial genome
genome mapping and counts of reads by gene locus are done with STAR; exact multihits are removed and count only consistent PE reads (in PE case).
Supplementary_files_format_and_content: type:count file (txt); content:GeneID,counts
|
| |
|
| Submission date |
Feb 09, 2021 |
| Last update date |
Apr 28, 2021 |
| Contact name |
Véronique BRUNAUD |
| E-mail(s) |
veronique.brunaud@inrae.fr
|
| Organization name |
INRAE - CNRS - UPSUD
|
| Lab |
IPS2
|
| Street address |
rue Noetzlin
|
| City |
Gif-sur-Yvette |
| ZIP/Postal code |
91190 |
| Country |
France |
| |
|
| Platform ID |
GPL19580 |
| Series (2) |
| GSE166447 |
RNaseI-RIPseq for rfl8-Functional characterization of the pentatricopeptide repeat protein PPR RFL8 |
| GSE166459 |
Nuclear compensation to a lineage-specific mitochondrial genomic rearrangement in plants |
|
| Relations |
| BioSample |
SAMN17841294 |
| SRA |
SRX10058393 |
