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Sample GSM507269 Query DataSets for GSM507269
Status Public on Mar 01, 2011
Title Dnmt3L heterozygous spermatids 1
Sample type RNA
 
Source name Mouse round spermatids
Organism Mus musculus
Characteristics strain: C57Bl/6
age: 8-12 weeks
tissue: Testis
genome/variation: Dnmt3L heterozygous
cell type: spermatids
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. RNA quality was assessed with Agilent Bioanalyser.
Label Cy3
Label protocol Standard Illumina protocol
 
Hybridization protocol Standard Illumina protocol
Scan protocol Standard Illumina protocol
Description no additional information
Data processing Un-normalized summary probe profiles were output from BeadStudio for two Illumina Mouse Whole-Genome Version 1.1 BeadChips. The raw intensities were transformed by variance stabilising transformation (vst) and quantile normalised using the lumi package of the Bioconductor open-source software project (http://www.bioconductor.org).
 
Submission date Feb 09, 2010
Last update date Mar 01, 2011
Contact name Gordon K Smyth
E-mail(s) smyth@wehi.edu.au
Phone (+61 3) 9345 2326
Fax (+61 3) 9347 0852
URL http://www.wehi.edu.au
Organization name Walter and Eliza Hall Institute of Medical Research
Department Bioinformatics
Lab Smyth
Street address 1G Royal Pde
City Parkville
State/province Vic
ZIP/Postal code 3052
Country Australia
 
Platform ID GPL6481
Series (1)
GSE20241 Genome-wide analysis of Dnmt3L heterozygous and wildtype spermatocytes and spermatids

Data table header descriptions
ID_REF
VALUE lumi (bioc) normalized log2 signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
102450270 6.658852608 0.87711
104200035 6.75076684 0.50094
100730138 6.871710349 0.20274
104230390 6.941097089 0.12414
102630735 6.733928561 0.58203
100450435 6.927949067 0.13724
101230594 6.714365791 0.67623
100780541 6.7472047 0.51778
104200184 6.662385365 0.8665
105360286 6.75330331 0.49407
103060411 6.741054535 0.54336
100630193 6.761967879 0.45976
105270286 6.653683153 0.89021
101690685 6.674100092 0.8403
100060180 6.878491903 0.19463
107100563 6.719567256 0.64941
100450451 6.674100092 0.8403
100610092 6.677163859 0.83468
104810452 13.39366088 0
6840019 9.581554367 0

Total number of rows: 46643

Table truncated, full table size 1266 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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