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Sample GSM507271 Query DataSets for GSM507271
Status Public on Mar 01, 2011
Title Dnmt3L heterozygous spermatids 3
Sample type RNA
 
Source name Mouse round spermatids
Organism Mus musculus
Characteristics strain: C57Bl/6
age: 8-12 weeks
tissue: Testis
genome/variation: Dnmt3L heterozygous
cell type: spermatids
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. RNA quality was assessed with Agilent Bioanalyser.
Label Cy3
Label protocol Standard Illumina protocol
 
Hybridization protocol Standard Illumina protocol
Scan protocol Standard Illumina protocol
Description no additional information
Data processing Un-normalized summary probe profiles were output from BeadStudio for two Illumina Mouse Whole-Genome Version 1.1 BeadChips. The raw intensities were transformed by variance stabilising transformation (vst) and quantile normalised using the lumi package of the Bioconductor open-source software project (http://www.bioconductor.org).
 
Submission date Feb 09, 2010
Last update date Mar 01, 2011
Contact name Gordon K Smyth
E-mail(s) smyth@wehi.edu.au
Phone (+61 3) 9345 2326
Fax (+61 3) 9347 0852
URL http://www.wehi.edu.au
Organization name Walter and Eliza Hall Institute of Medical Research
Department Bioinformatics
Lab Smyth
Street address 1G Royal Pde
City Parkville
State/province Vic
ZIP/Postal code 3052
Country Australia
 
Platform ID GPL6481
Series (1)
GSE20241 Genome-wide analysis of Dnmt3L heterozygous and wildtype spermatocytes and spermatids

Data table header descriptions
ID_REF
VALUE lumi (bioc) normalized log2 signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
102450270 6.67369458 0.8403
104200035 6.850507627 0.25764
100730138 6.778204776 0.41859
104230390 7.082160361 0.05739
102630735 6.684466615 0.81347
100450435 6.92451084 0.14972
101230594 6.757967399 0.4922
100780541 6.759594407 0.48534
104200184 6.713403852 0.69308
105360286 6.713403852 0.6937
103060411 6.774082948 0.43231
100630193 6.650504395 0.89832
105270286 6.719463411 0.67249
101690685 6.637067588 0.9214
100060180 6.76766948 0.45041
107100563 6.725492447 0.6413
100450451 6.648630261 0.90206
100610092 6.755830878 0.50468
104810452 13.02575675 0
6840019 9.399031042 0

Total number of rows: 46643

Table truncated, full table size 1267 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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