|
| Status |
Public on Feb 22, 2021 |
| Title |
w1118-fed-rep1 |
| Sample type |
RNA |
| |
|
| Source name |
males, whole body, 5-days old, fed
|
| Organism |
Drosophila melanogaster |
| Characteristics |
tissue: whole body
genotype: w1118
Sex: male
age: 5 days after eclosion
treatment: Fed
|
| Treatment protocol |
Flies were grown for 4 days on regular food. Then, flies were either left on regular food, or placed into empty vials with moist whatman paper for 24 hours.
|
| Growth protocol |
Flies were grown at standard conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Whole bodies of ten males were used to extract total RNA. RNA was extracted using ZR Tissue and Insect RNA MicroPrep™ (Zymo Research, #R2030) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotin labeling was performed using the WT Plus reagent kit (Affymetrix, Santa Clara, CA) according to the manufacturer's protocol.
|
| |
|
| Hybridization protocol |
The labeled, fragmented DNA was hybridized to the Affymetrix Drosophila Gene 1.0 ST array for 18 hours in a GeneChip Hybridization oven 640 at 45oC with rotation (60 rpm). The hybridized samples were washed and stained using an Affymetrix fluidics station 450.
|
| Scan protocol |
After staining, microarrays were immediately scanned using an Affymetrix GeneArray Scanner 3000 7G Plus.
|
| Description |
wt-F-1
Gene expression data for w1118 fed male flies.
|
| Data processing |
Raw Affymetrix CEL files were normalized to produce Entrez-Gene-identifier-specific expression values using the implementation of the Robust Multiarray Average (RMA) in the affy Bioconductor package (version 1.48.0), using R version 3.2.0 and the Brainarray drogene10stdmentrezgcdf R package (version 20.0.0).
|
| |
|
| Submission date |
Feb 21, 2021 |
| Last update date |
Feb 22, 2021 |
| Contact name |
Katarina Akhmetova |
| E-mail(s) |
katarina@uab.edu
|
| Organization name |
University of Alabama at Birmingham
|
| Street address |
720 20th St S, Kaul 548
|
| City |
Birmingham |
| State/province |
AL |
| ZIP/Postal code |
35233 |
| Country |
USA |
| |
|
| Platform ID |
GPL29751 |
| Series (1) |
| GSE167164 |
Expression data for dSTING mutant vs. wild type flies |
|
