|
| Status |
Public on Feb 23, 2021 |
| Title |
Treatment group 1-rep 1-Nk1.1- |
| Sample type |
RNA |
| |
|
| Source name |
SPLENOCYTES
|
| Organism |
Mus musculus |
| Characteristics |
cells: CD8+CD69+NK1.1- T cells
treatment: Double Loaded DC + Drug
|
| Treatment protocol |
CD8+CD69+NK1.1+ and CD8+CD69+NK1.1- T cells were sorted by flow cytometry from mouse splenocytes antigenically stimulated overnight.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from the cells with the RNeasy Mini Kit (Qiagen), according to the manufacturer’s instructions. Inclusion of 10 mL/L of the RNase inhibitor NucleoGuard (AmpTec) in the lysis buffer improved RNA yield and recoveries of long transcripts. All samples were treated with TURBO DNA-free (Ambion) to remove trace amounts of genomic DNA.
|
| Label |
biotin
|
| Label protocol |
Single-stranded cDNA was generated from the amplified cRNA with the WT cDNA Synthesis Kit (Affymetrix) and then fragmented and labeled with the WT Terminal Labeling Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
Samples were hybridized with GeneChip mouse WT Pico-Clariom D Exon Arrays (Affymetrix) and scanned at the Sequencing and Microarray Facility at the University of Texas MDAnderson Cancer Center.
|
| Scan protocol |
Array scanning was performed according to the manufacturer's instruction (Affymetrix)
|
| Description |
RMA expression value derived from Transcriptome Analysis Console v3.0
|
| Data processing |
Raw data were processed with Transcriptome Analysis Console v3.0 (Affymetrix). Data analysis and statistical evaluations were performed with R version 3.4.3. Class comparison paired T test was used with an FDR of 0.05% to 0.1%.
|
| |
|
| Submission date |
Feb 22, 2021 |
| Last update date |
Feb 23, 2021 |
| Contact name |
vanaja konduri |
| E-mail(s) |
konduri@bcm.edu
|
| Phone |
7137986413
|
| Organization name |
Baylor College of Medicine
|
| Department |
Pathology&Immunology
|
| Lab |
N911
|
| Street address |
One Baylor Plaza
|
| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platform ID |
GPL20775 |
| Series (1) |
| GSE167276 |
Exon Level Expression Profiling: a Novel Unbiased Transcriptome (Mouse) |
|
