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Status |
Public on May 01, 2022 |
Title |
Day0-3 (miRNA-seq) |
Sample type |
SRA |
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Source name |
whole body
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Organism |
Aedes aegypti |
Characteristics |
strain: Rockefeller tissue: whole body protocol: blood feeding blood from: human donor 3 time: 1 hr
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Treatment protocol |
7 days old mosquitos were following a blood meal, then cultured after 1hr or 6 days
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Growth protocol |
Aedes Aegypti was reared at 28℃ and 80% humidity.
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Extracted molecule |
total RNA |
Extraction protocol |
The total mosquito RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries. RNA libraries were prepared for sequencing using standard Illumina protocols small RNA-Seq
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Library strategy |
miRNA-Seq |
Library source |
transcriptomic |
Library selection |
size fractionation |
Instrument model |
BGISEQ-500 |
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Data processing |
Remove low quality tags Remove tags with 5' primer contaminants Remove tags without 3' primer Remove tags without insertion Remove tags with poly A Remove tags shorter than 18nt Genome_build: miRBase 22.1 and Rfam 14.4 Supplementary_files_format_and_content: Excel files include RPKM values, log2 ratio, P values, FDR, KEGG Orthology, and GO analysis for each Sample ...
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Submission date |
Feb 23, 2021 |
Last update date |
May 01, 2022 |
Contact name |
Yibin Zhu |
E-mail(s) |
zhuyibin1989@126.com
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Phone |
18515653980
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Organization name |
Tsinghua University
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Street address |
Tsinghua, Beijing, China
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City |
Beijing |
ZIP/Postal code |
100084 |
Country |
China |
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Platform ID |
GPL29762 |
Series (2) |
GSE167346 |
A human blood-derived microRNA facilitates flavivirus infection in fed mosquitoes [miRNA-seq] |
GSE167348 |
A human blood-derived microRNA facilitates flavivirus infection in fed mosquitoes |
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Relations |
BioSample |
SAMN18038668 |
SRA |
SRX10157099 |
Supplementary file |
Size |
Download |
File type/resource |
GSM5102590_Day0-3.expr.txt.gz |
17.4 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
Processed data are available on Series record |
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