|
| Status |
Public on Nov 15, 2023 |
| Title |
Forelimb_15 |
| Sample type |
SRA |
| |
|
| Source name |
Presumptive forelimb mesenchyme (HH15)
|
| Organism |
Gallus gallus |
| Characteristics |
cell type: Mesenchyme cells
tissue: Presumptive forelimb
|
| Treatment protocol |
N/A
|
| Growth protocol |
Directly dissected from chick embryos from all stages
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
polyadenylated RNA was extracted by mRNA purification kit by Qiagen
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Data processing |
After bclfastq processing to demultiplex paired-end library, Tophat (version 1.4.0) was used for alignment.
Genome_build: galGal4
Supplementary_files_format_and_content: tab-delimited text file with read counts and fold change, p-value comparison
|
| |
|
| Submission date |
Feb 24, 2021 |
| Last update date |
Nov 15, 2023 |
| Contact name |
CHANGHEE LEE |
| E-mail(s) |
chlee@genetics.med.harvard.edu
|
| Phone |
617-432-6534
|
| Organization name |
Harvard Medical School
|
| Department |
Genetics
|
| Lab |
Clifford Tabin
|
| Street address |
77 AVENUE LOUIS PASTEUR
|
| City |
BOSTON |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platform ID |
GPL19005 |
| Series (2) |
| GSE167419 |
Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors [bulkRNA-seq] |
| GSE167423 |
Direct Reprogramming of Non-limb Fibroblasts to Cells with Properties of Limb Progenitors |
|
| Relations |
| BioSample |
SAMN18050575 |
| SRA |
SRX10162895 |
