|
| Status |
Public on Feb 23, 2024 |
| Title |
M337V-Rep1 |
| Sample type |
SRA |
| |
|
| Source name |
iPS Cell Line
|
| Organism |
Homo sapiens |
| Characteristics |
genotype/variation: M337V TDP-43 mutation
|
| Treatment protocol |
Cells were cultured in mTeSR1 media (STEMCELL Technologies) on matrigel-coated plates.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from TDP-43 (WT) and TDP-43(M337V) ALS iPSCs using RNeasy mini kit (qiagen) according to manufacturer's protocol
Library was prepared using Illumina TrueSeq kit
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2000 |
| |
|
| Description |
Patient derived induced pluripotent Cell Line with M337V TDP-43 mutation
TDP-43(M337V) mutant cell line
|
| Data processing |
Images were analyzed using OLB 1.9 and CASAVA_v1.8.2
TopHat v2.0.9 (bowtie2/2.1.0) was used to map the sequences to human genome hg19
Reads counts were processed by DEXSeq v 1.10.6
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include exon level count values for each Sample
|
| |
|
| Submission date |
Feb 25, 2021 |
| Last update date |
Feb 23, 2024 |
| Contact name |
Ashish Misra |
| E-mail(s) |
ashishmisra@bt.iith.ac.in
|
| Organization name |
Indian Institute of Technology Hyderabad
|
| Department |
Biotechnology
|
| Street address |
Kandi
|
| City |
Sangareddy |
| State/province |
Telangana |
| ZIP/Postal code |
502285 |
| Country |
India |
| |
|
| Platform ID |
GPL11154 |
| Series (1) |
| GSE167557 |
Transcriptome profiling (RNA-seq) to identify aberrant splicing events in induced pluripotent stem cells derived from an ALS patient harboring a TDP-43(M337V) mutation |
|
| Relations |
| BioSample |
SAMN18059078 |
| SRA |
SRX10170767 |
