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Sample GSM5113995 Query DataSets for GSM5113995
Status Public on Nov 01, 2022
Title control
Sample type RNA
 
Source name macrophages_control
Organism Mus musculus
Characteristics strain: C57BL6
tissue: bone marrow
cell type: macrophages
Treatment protocol Bone marrow derived macrophages were treated with or without LPS.
Growth protocol Bone marrow derived macrophages were cultured with RPMI containing 10% FBS and MCSF.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted with Nucleospin RNA plus kit
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
 
Hybridization protocol Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description Gene expression data from bone marrow derived macrophages
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
 
Submission date Feb 26, 2021
Last update date Nov 01, 2022
Contact name Tomohiko Murakami
E-mail(s) murakami.tomohiko.dent@osaka-u.ac.jp
Phone 81668792887
Organization name Osaka University
Street address 1-8 Yamada-Oka
City Suita
State/province OSAKA
ZIP/Postal code 565-0871
Country Japan
 
Platform ID GPL1261
Series (1)
GSE167853 Expression data from mouse bone marrow derived macrophages

Data table header descriptions
ID_REF
VALUE MAS5.0
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 209.452 P 0.000340305
AFFX-BioB-M_at 283.033 P 4.42873e-05
AFFX-BioB-3_at 204.953 P 5.16732e-05
AFFX-BioC-5_at 633.925 P 5.16732e-05
AFFX-BioC-3_at 878.095 P 4.42873e-05
AFFX-BioDn-5_at 1552.97 P 4.42873e-05
AFFX-BioDn-3_at 2721.59 P 8.14279e-05
AFFX-CreX-5_at 7858.69 P 5.16732e-05
AFFX-CreX-3_at 8402.48 P 4.42873e-05
AFFX-DapX-5_at 706.349 P 4.42873e-05
AFFX-DapX-M_at 1343.41 P 0.000169227
AFFX-DapX-3_at 1031.4 P 4.42873e-05
AFFX-LysX-5_at 56.9695 P 0.000146581
AFFX-LysX-M_at 95.754 P 0.0151826
AFFX-LysX-3_at 156.157 P 9.4506e-05
AFFX-PheX-5_at 82.2437 P 0.000258358
AFFX-PheX-M_at 109.977 P 5.16732e-05
AFFX-PheX-3_at 161.398 P 7.00668e-05
AFFX-ThrX-5_at 84.3794 P 0.000445901
AFFX-ThrX-M_at 149.324 P 7.00668e-05

Total number of rows: 45101

Table truncated, full table size 1382 Kbytes.




Supplementary file Size Download File type/resource
GSM5113995_01_control_Mouse430_2_.CEL.gz 3.6 Mb (ftp)(http) CEL
GSM5113995_01_control_Mouse430_2_.mas5.CHP.gz 246.8 Kb (ftp)(http) CHP
Processed data included within Sample table

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