|
Status |
Public on Nov 01, 2022 |
Title |
control |
Sample type |
RNA |
|
|
Source name |
macrophages_control
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL6 tissue: bone marrow cell type: macrophages
|
Treatment protocol |
Bone marrow derived macrophages were treated with or without LPS.
|
Growth protocol |
Bone marrow derived macrophages were cultured with RPMI containing 10% FBS and MCSF.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted with Nucleospin RNA plus kit
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA.
|
|
|
Hybridization protocol |
Following fragmentation, 12.5 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Mouse Genome 430 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using the GeneChip Scanner 3000 7G.
|
Description |
Gene expression data from bone marrow derived macrophages
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings.
|
|
|
Submission date |
Feb 26, 2021 |
Last update date |
Nov 01, 2022 |
Contact name |
Tomohiko Murakami |
E-mail(s) |
murakami.tomohiko.dent@osaka-u.ac.jp
|
Phone |
81668792887
|
Organization name |
Osaka University
|
Street address |
1-8 Yamada-Oka
|
City |
Suita |
State/province |
OSAKA |
ZIP/Postal code |
565-0871 |
Country |
Japan |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE167853 |
Expression data from mouse bone marrow derived macrophages |
|